A simple visual ethanol biosensor based on alcohol oxidase immobilized onto polyaniline film for halal verification of fermented beverage samples.

Bambang Kuswandi, Titi Irmawati, Moch Amrun Hidayat, Jayus, Musa Ahmad

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A simple visual ethanol biosensor based on alcohol oxidase (AOX) immobilised onto polyaniline (PANI) film for halal verification of fermented beverage samples is described. This biosensor responds to ethanol via a colour change from green to blue, due to the enzymatic reaction of ethanol that produces acetaldehyde and hydrogen peroxide, when the latter oxidizes the PANI film. The procedure to obtain this biosensor consists of the immobilization of AOX onto PANI film by adsorption. For the immobilisation, an AOX solution is deposited on the PANI film and left at room temperature until dried (30 min). The biosensor was constructed as a dip stick for visual and simple use. The colour changes of the films have been scanned and analysed using image analysis software (i.e., ImageJ) to study the characteristics of the biosensor's response toward ethanol. The biosensor has a linear response in an ethanol concentration range of 0.01%-0.8%, with a correlation coefficient (r) of 0.996. The limit detection of the biosensor was 0.001%, with reproducibility (RSD) of 1.6% and a life time up to seven weeks when stored at 4 °C. The biosensor provides accurate results for ethanol determination in fermented drinks and was in good agreement with the standard method (gas chromatography) results. Thus, the biosensor could be used as a simple visual method for ethanol determination in fermented beverage samples that can be useful for Muslim community for halal verification.

Original languageEnglish
Pages (from-to)2135-2149
Number of pages15
JournalSensors (Basel, Switzerland)
Volume14
Issue number2
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

alcohol oxidase
beverages
Beverages
oxidase
Biosensing Techniques
Polyaniline
bioinstrumentation
Biosensors
alcohols
Alcohols
Ethanol
ethyl alcohol
immobilization
Immobilization
Color
color
polyaniline
Oxidoreductases
Islam
Acetaldehyde

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A simple visual ethanol biosensor based on alcohol oxidase immobilized onto polyaniline film for halal verification of fermented beverage samples. / Kuswandi, Bambang; Irmawati, Titi; Hidayat, Moch Amrun; Jayus, ; Ahmad, Musa.

In: Sensors (Basel, Switzerland), Vol. 14, No. 2, 2014, p. 2135-2149.

Research output: Contribution to journalArticle

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