A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes

Nur Ellina Azmi, Noor Izaanin Ramli, Jaafar Abdullah, Muhammad Azmi Abdul Hamid, Hamidah Sidek, Samsulida Abd Rahman, Nurhayati Ariffin, Nor Azah Yusof

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

A novel optical detection system consisting of combination of uricase/HRP-CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125-1000. μM with detection limit of 125.

Original languageEnglish
Pages (from-to)129-133
Number of pages5
JournalBiosensors and Bioelectronics
Volume67
DOIs
Publication statusPublished - 5 May 2015

Fingerprint

Quantum Dots
Biosensing Techniques
Urate Oxidase
Uric Acid
Biosensors
Semiconductor quantum dots
Horseradish Peroxidase
Enzymes
Fluorescence
Acids
Hydrogen peroxide
Hydrogen Peroxide
Optical Devices
Limit of Detection
Urine

Keywords

  • Enzyme
  • Fluorescence
  • Quantum dots
  • Quenching
  • Uric acid

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes. / Azmi, Nur Ellina; Ramli, Noor Izaanin; Abdullah, Jaafar; Abdul Hamid, Muhammad Azmi; Sidek, Hamidah; Abd Rahman, Samsulida; Ariffin, Nurhayati; Yusof, Nor Azah.

In: Biosensors and Bioelectronics, Vol. 67, 05.05.2015, p. 129-133.

Research output: Contribution to journalArticle

Azmi, Nur Ellina ; Ramli, Noor Izaanin ; Abdullah, Jaafar ; Abdul Hamid, Muhammad Azmi ; Sidek, Hamidah ; Abd Rahman, Samsulida ; Ariffin, Nurhayati ; Yusof, Nor Azah. / A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes. In: Biosensors and Bioelectronics. 2015 ; Vol. 67. pp. 129-133.
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