A simple and effective isocratic HPLC method for fast identification and quantification of surfactin

Muhammad Qadri Effendy Mubarak, Abdul Rahman Hassan, Aidil Abdul Hamid, Sahaid Khalil, Mohd Hafez Mohd Isa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of this study was to establish a simple, accurate and reproducible method for the identification and quantification of surfactin using high-performance liquid chromatography (HPLC). Previously reported method of identification and quantification of surfactin were time consuming and requires a large quantity of mobile phase. The new method was achieved by application of Chromolith® high performance RP-18 (100 × 4.6 mm, 5 μm) as the stationary phase and optimization of mobile phase ratio and flow rate. Mobile phase consisted of acetonitrile (ACN) and 3.8 mM trifluroacetic acid (TFA) solution of 80:20 ratio at flow rate of 2.2 mL/min was obtained as the optimal conditions. Total elution time of the obtained surfactin peaks was four times quicker than various methods previously reported in the literature. The method described here allowed for fine separation of surfactin in standard sample (98% purity) and surfactin in fermentation broth.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalSains Malaysiana
Volume44
Issue number1
Publication statusPublished - 1 Jan 2015

Fingerprint

High performance liquid chromatography
Flow rate
Fermentation
Acids
acetonitrile

Keywords

  • High-performance liquid chromatography (HPLC)
  • Isocratic
  • Mobile phase
  • Stationary phase
  • Surfactin

ASJC Scopus subject areas

  • General

Cite this

Mubarak, M. Q. E., Hassan, A. R., Abdul Hamid, A., Khalil, S., & Isa, M. H. M. (2015). A simple and effective isocratic HPLC method for fast identification and quantification of surfactin. Sains Malaysiana, 44(1), 115-120.

A simple and effective isocratic HPLC method for fast identification and quantification of surfactin. / Mubarak, Muhammad Qadri Effendy; Hassan, Abdul Rahman; Abdul Hamid, Aidil; Khalil, Sahaid; Isa, Mohd Hafez Mohd.

In: Sains Malaysiana, Vol. 44, No. 1, 01.01.2015, p. 115-120.

Research output: Contribution to journalArticle

Mubarak, Muhammad Qadri Effendy ; Hassan, Abdul Rahman ; Abdul Hamid, Aidil ; Khalil, Sahaid ; Isa, Mohd Hafez Mohd. / A simple and effective isocratic HPLC method for fast identification and quantification of surfactin. In: Sains Malaysiana. 2015 ; Vol. 44, No. 1. pp. 115-120.
@article{2947511ec2234cefb00f9a695b5821e4,
title = "A simple and effective isocratic HPLC method for fast identification and quantification of surfactin",
abstract = "The aim of this study was to establish a simple, accurate and reproducible method for the identification and quantification of surfactin using high-performance liquid chromatography (HPLC). Previously reported method of identification and quantification of surfactin were time consuming and requires a large quantity of mobile phase. The new method was achieved by application of Chromolith{\circledR} high performance RP-18 (100 × 4.6 mm, 5 μm) as the stationary phase and optimization of mobile phase ratio and flow rate. Mobile phase consisted of acetonitrile (ACN) and 3.8 mM trifluroacetic acid (TFA) solution of 80:20 ratio at flow rate of 2.2 mL/min was obtained as the optimal conditions. Total elution time of the obtained surfactin peaks was four times quicker than various methods previously reported in the literature. The method described here allowed for fine separation of surfactin in standard sample (98{\%} purity) and surfactin in fermentation broth.",
keywords = "High-performance liquid chromatography (HPLC), Isocratic, Mobile phase, Stationary phase, Surfactin",
author = "Mubarak, {Muhammad Qadri Effendy} and Hassan, {Abdul Rahman} and {Abdul Hamid}, Aidil and Sahaid Khalil and Isa, {Mohd Hafez Mohd}",
year = "2015",
month = "1",
day = "1",
language = "English",
volume = "44",
pages = "115--120",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "1",

}

TY - JOUR

T1 - A simple and effective isocratic HPLC method for fast identification and quantification of surfactin

AU - Mubarak, Muhammad Qadri Effendy

AU - Hassan, Abdul Rahman

AU - Abdul Hamid, Aidil

AU - Khalil, Sahaid

AU - Isa, Mohd Hafez Mohd

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The aim of this study was to establish a simple, accurate and reproducible method for the identification and quantification of surfactin using high-performance liquid chromatography (HPLC). Previously reported method of identification and quantification of surfactin were time consuming and requires a large quantity of mobile phase. The new method was achieved by application of Chromolith® high performance RP-18 (100 × 4.6 mm, 5 μm) as the stationary phase and optimization of mobile phase ratio and flow rate. Mobile phase consisted of acetonitrile (ACN) and 3.8 mM trifluroacetic acid (TFA) solution of 80:20 ratio at flow rate of 2.2 mL/min was obtained as the optimal conditions. Total elution time of the obtained surfactin peaks was four times quicker than various methods previously reported in the literature. The method described here allowed for fine separation of surfactin in standard sample (98% purity) and surfactin in fermentation broth.

AB - The aim of this study was to establish a simple, accurate and reproducible method for the identification and quantification of surfactin using high-performance liquid chromatography (HPLC). Previously reported method of identification and quantification of surfactin were time consuming and requires a large quantity of mobile phase. The new method was achieved by application of Chromolith® high performance RP-18 (100 × 4.6 mm, 5 μm) as the stationary phase and optimization of mobile phase ratio and flow rate. Mobile phase consisted of acetonitrile (ACN) and 3.8 mM trifluroacetic acid (TFA) solution of 80:20 ratio at flow rate of 2.2 mL/min was obtained as the optimal conditions. Total elution time of the obtained surfactin peaks was four times quicker than various methods previously reported in the literature. The method described here allowed for fine separation of surfactin in standard sample (98% purity) and surfactin in fermentation broth.

KW - High-performance liquid chromatography (HPLC)

KW - Isocratic

KW - Mobile phase

KW - Stationary phase

KW - Surfactin

UR - http://www.scopus.com/inward/record.url?scp=84922247756&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922247756&partnerID=8YFLogxK

M3 - Article

VL - 44

SP - 115

EP - 120

JO - Sains Malaysiana

JF - Sains Malaysiana

SN - 0126-6039

IS - 1

ER -