A restrictive virus tropism, latency and reactivation of pseudorabies virus following irreversible deletion of BsrI restriction site in the thymidine-kinase gene

Mohd Lila Mohd Azmi, Nazariah Allaudin Zeenathul, Abdel Wahid Saeed Ali, Che Abd. Rahim Mohamed, Awang Isa Kamarudin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

At the dose of 1000 p.f.u. per mouse, 100% mortality occurred in mice inoculated with wild-type pseudorabies virus (PrV). In contrast, upon stable deletion of 10 bp nucleotides at the BsrI site within the TK gene, PrV was rendered to be completely apathogenic. The deletion also caused the virus to be less capable of replicating in respiratory as well as in nervous system tissues. Although animals were exposed to high titers of TK-deleted PrVs, the virus failed to replicate to a high titer as compared to the pathogenic parental virus. In contrast to previous studies, the deletion in the TK gene did not prevent the virus from establishing latency. Upon immunosuppression, the latent virus, however, reactivated but replicated at low titers. Interestingly, TK-deleted virus established latency and reactivation, that are occurred only in trigeminal ganglia and the cerebrum, and no other tissues involved. Following reactivation, there was no indication of virus shedding in respiratory tissues as confirmed by virus isolation and polymerase chain reaction (PCR) technique targeting at the gB gene of PrV. The non-pathogenic virus with non-shedding characteristics, upon reactivation of the latent virus, would be the important feature of a live virus vaccine candidate.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalJournal of Microbiology
Volume40
Issue number1
Publication statusPublished - Mar 2002
Externally publishedYes

Fingerprint

Virus Latency
Suid Herpesvirus 1
Tropism
Thymidine Kinase
Viruses
Genes
Virus Shedding
Nerve Tissue
Trigeminal Ganglion
Cerebrum
Immunosuppression
Nervous System
Vaccines
Nucleotides
Polymerase Chain Reaction
Mortality

Keywords

  • Latency
  • Pseudorabies virus
  • Reactivation
  • Thymidine kinase
  • Tropism

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

A restrictive virus tropism, latency and reactivation of pseudorabies virus following irreversible deletion of BsrI restriction site in the thymidine-kinase gene. / Azmi, Mohd Lila Mohd; Zeenathul, Nazariah Allaudin; Ali, Abdel Wahid Saeed; Mohamed, Che Abd. Rahim; Kamarudin, Awang Isa.

In: Journal of Microbiology, Vol. 40, No. 1, 03.2002, p. 1-10.

Research output: Contribution to journalArticle

Azmi, Mohd Lila Mohd ; Zeenathul, Nazariah Allaudin ; Ali, Abdel Wahid Saeed ; Mohamed, Che Abd. Rahim ; Kamarudin, Awang Isa. / A restrictive virus tropism, latency and reactivation of pseudorabies virus following irreversible deletion of BsrI restriction site in the thymidine-kinase gene. In: Journal of Microbiology. 2002 ; Vol. 40, No. 1. pp. 1-10.
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