A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination

Alizar Ulianas, Yook Heng Lee, Musa Ahmad, Han Yih Lau, Zamri Ishak, Ling Ling Tan @ Chong

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A regenerable electrochemical DNA biosensor based on a new type of acrylic microspheres and gold nanoparticles (AuNPs) composite coated onto a screen printed electrode (SPE) has been successfully developed for specific determination of the 35 S promoter from cauliflower mosaic virus (CaMV 35S) gene in soybean. DNA probe was immobilised onto acrylic microspheres via covalent bonding. The presence of modified gene in soybean can be detected via hybridisation of CaMV 35S gene-modified DNA with immobilised DNA probe, which was monitored by differential pulse voltammetry of anthraquinone-2-sulfonic acid monohydrate sodium salt (AQMS) as redox indicator during hybridisation event. The peak current signal of AQMS was linearly related to the target CaMV 35S gene concentration over the range of 2 × 10-15 to 2 × 10 -9 M (R2 = 0.982) with a very low concentration detect limit (7.79 × 10-16 M). The recovery test showed satisfactory results of 94.6 ± 5.1-105.4 ± 4.9% (n = 5) when the biosensor was used for the determination of genetically modified (GM) DNA sequences extracted from GM soybean samples. The DNA biosensor showed good reproducibility (relative standard deviation (RSD) below 5.0%, n = 5) and regenerability (RSD below 5.0%, n = 7). The biosensor response was stable up to 45 days of storage period at 4 C. The main advantages of this biosensor design are very low detection limit and capability of reusing the biosensor for at least seven times after regeneration with mild sodium hydroxide.

Original languageEnglish
Pages (from-to)694-701
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume190
DOIs
Publication statusPublished - 2014

Fingerprint

soybeans
Microspheres
bioinstrumentation
Biosensors
Gold
Acrylics
DNA
deoxyribonucleic acid
gold
Nanoparticles
genes
nanoparticles
composite materials
Composite materials
Genes
DNA Probes
standard deviation
Sodium
Immobilized Nucleic Acids
Sodium Hydroxide

Keywords

  • CaMV 35S promoter
  • Food GM DNA
  • GM DNA biosensor
  • Microspheres

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

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title = "A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination",
abstract = "A regenerable electrochemical DNA biosensor based on a new type of acrylic microspheres and gold nanoparticles (AuNPs) composite coated onto a screen printed electrode (SPE) has been successfully developed for specific determination of the 35 S promoter from cauliflower mosaic virus (CaMV 35S) gene in soybean. DNA probe was immobilised onto acrylic microspheres via covalent bonding. The presence of modified gene in soybean can be detected via hybridisation of CaMV 35S gene-modified DNA with immobilised DNA probe, which was monitored by differential pulse voltammetry of anthraquinone-2-sulfonic acid monohydrate sodium salt (AQMS) as redox indicator during hybridisation event. The peak current signal of AQMS was linearly related to the target CaMV 35S gene concentration over the range of 2 × 10-15 to 2 × 10 -9 M (R2 = 0.982) with a very low concentration detect limit (7.79 × 10-16 M). The recovery test showed satisfactory results of 94.6 ± 5.1-105.4 ± 4.9{\%} (n = 5) when the biosensor was used for the determination of genetically modified (GM) DNA sequences extracted from GM soybean samples. The DNA biosensor showed good reproducibility (relative standard deviation (RSD) below 5.0{\%}, n = 5) and regenerability (RSD below 5.0{\%}, n = 7). The biosensor response was stable up to 45 days of storage period at 4 C. The main advantages of this biosensor design are very low detection limit and capability of reusing the biosensor for at least seven times after regeneration with mild sodium hydroxide.",
keywords = "CaMV 35S promoter, Food GM DNA, GM DNA biosensor, Microspheres",
author = "Alizar Ulianas and Lee, {Yook Heng} and Musa Ahmad and Lau, {Han Yih} and Zamri Ishak and {Tan @ Chong}, {Ling Ling}",
year = "2014",
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T1 - A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination

AU - Ulianas, Alizar

AU - Lee, Yook Heng

AU - Ahmad, Musa

AU - Lau, Han Yih

AU - Ishak, Zamri

AU - Tan @ Chong, Ling Ling

PY - 2014

Y1 - 2014

N2 - A regenerable electrochemical DNA biosensor based on a new type of acrylic microspheres and gold nanoparticles (AuNPs) composite coated onto a screen printed electrode (SPE) has been successfully developed for specific determination of the 35 S promoter from cauliflower mosaic virus (CaMV 35S) gene in soybean. DNA probe was immobilised onto acrylic microspheres via covalent bonding. The presence of modified gene in soybean can be detected via hybridisation of CaMV 35S gene-modified DNA with immobilised DNA probe, which was monitored by differential pulse voltammetry of anthraquinone-2-sulfonic acid monohydrate sodium salt (AQMS) as redox indicator during hybridisation event. The peak current signal of AQMS was linearly related to the target CaMV 35S gene concentration over the range of 2 × 10-15 to 2 × 10 -9 M (R2 = 0.982) with a very low concentration detect limit (7.79 × 10-16 M). The recovery test showed satisfactory results of 94.6 ± 5.1-105.4 ± 4.9% (n = 5) when the biosensor was used for the determination of genetically modified (GM) DNA sequences extracted from GM soybean samples. The DNA biosensor showed good reproducibility (relative standard deviation (RSD) below 5.0%, n = 5) and regenerability (RSD below 5.0%, n = 7). The biosensor response was stable up to 45 days of storage period at 4 C. The main advantages of this biosensor design are very low detection limit and capability of reusing the biosensor for at least seven times after regeneration with mild sodium hydroxide.

AB - A regenerable electrochemical DNA biosensor based on a new type of acrylic microspheres and gold nanoparticles (AuNPs) composite coated onto a screen printed electrode (SPE) has been successfully developed for specific determination of the 35 S promoter from cauliflower mosaic virus (CaMV 35S) gene in soybean. DNA probe was immobilised onto acrylic microspheres via covalent bonding. The presence of modified gene in soybean can be detected via hybridisation of CaMV 35S gene-modified DNA with immobilised DNA probe, which was monitored by differential pulse voltammetry of anthraquinone-2-sulfonic acid monohydrate sodium salt (AQMS) as redox indicator during hybridisation event. The peak current signal of AQMS was linearly related to the target CaMV 35S gene concentration over the range of 2 × 10-15 to 2 × 10 -9 M (R2 = 0.982) with a very low concentration detect limit (7.79 × 10-16 M). The recovery test showed satisfactory results of 94.6 ± 5.1-105.4 ± 4.9% (n = 5) when the biosensor was used for the determination of genetically modified (GM) DNA sequences extracted from GM soybean samples. The DNA biosensor showed good reproducibility (relative standard deviation (RSD) below 5.0%, n = 5) and regenerability (RSD below 5.0%, n = 7). The biosensor response was stable up to 45 days of storage period at 4 C. The main advantages of this biosensor design are very low detection limit and capability of reusing the biosensor for at least seven times after regeneration with mild sodium hydroxide.

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