A quantification strategy for DNA hybridization via measurement of adsorbed anthraquinone monosulphonic acid on silica nanospheres

Yew Pei Ling, Yook Heng Lee, Hui Yee Chee

Research output: Contribution to journalArticle

Abstract

An amperometric DNA biosensor was developed based on a DNA quantification concept involving the adsorption behavior of anthraquinone monosulphonic acid (AQMS). The biosensor was built from a carbon pasted screen-printed electrode coated with silica nanospheres and gold nanoparticles. Silica nanospheres of 65–84 nm were synthesized via sonication and its surface further modified with amine group. Aminated silica nanospheres immobilize amine-functionalized DNA probe covalently via glutaraldehyde linkers while gold nanoparticles used as an effective electron transfer agent. Anionic AQMS can physically adsorb onto cationic aminated silica nanospheres, and the total AQMS adsorption will be reduced if hybridization occurs. Hence, an increasing amount of hybridized DNA cause a declination in differential pulse voltammetric current signal of AQMS at −0.50 V. The biosensor yielded a linear response range of 10−9–0.5 μM (R2 = 0.97) towards DNA target and showed negligible response to mismatched and noncomplemetary DNA. The fabricated DNA biosensor as an early detection and possible noninvasive method for clinical and medical use was successfully performed; with the detection of synthetic DNA target and cDNA that were reverse-transcripted from dengue virus RNA.

Original languageEnglish
Pages (from-to)640-650
Number of pages11
JournalMeasurement: Journal of the International Measurement Confederation
Volume135
DOIs
Publication statusPublished - 1 Mar 2019

Fingerprint

anthraquinones
Nanospheres
DNA
deoxyribonucleic acid
Silica
silicon dioxide
acids
Acids
bioinstrumentation
Biosensors
Amines
amines
Gold
gold
Nanoparticles
Adsorption
nanoparticles
adsorption
Sonication
declination

Keywords

  • Anthraquinone monosulphonic acid
  • Dengue infection detection
  • DNA biosensor
  • Probe hybridization
  • Silica nanomaterials

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

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title = "A quantification strategy for DNA hybridization via measurement of adsorbed anthraquinone monosulphonic acid on silica nanospheres",
abstract = "An amperometric DNA biosensor was developed based on a DNA quantification concept involving the adsorption behavior of anthraquinone monosulphonic acid (AQMS). The biosensor was built from a carbon pasted screen-printed electrode coated with silica nanospheres and gold nanoparticles. Silica nanospheres of 65–84 nm were synthesized via sonication and its surface further modified with amine group. Aminated silica nanospheres immobilize amine-functionalized DNA probe covalently via glutaraldehyde linkers while gold nanoparticles used as an effective electron transfer agent. Anionic AQMS can physically adsorb onto cationic aminated silica nanospheres, and the total AQMS adsorption will be reduced if hybridization occurs. Hence, an increasing amount of hybridized DNA cause a declination in differential pulse voltammetric current signal of AQMS at −0.50 V. The biosensor yielded a linear response range of 10−9–0.5 μM (R2 = 0.97) towards DNA target and showed negligible response to mismatched and noncomplemetary DNA. The fabricated DNA biosensor as an early detection and possible noninvasive method for clinical and medical use was successfully performed; with the detection of synthetic DNA target and cDNA that were reverse-transcripted from dengue virus RNA.",
keywords = "Anthraquinone monosulphonic acid, Dengue infection detection, DNA biosensor, Probe hybridization, Silica nanomaterials",
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