A purified novel protein from the culture media of Chlorella vulgaris inhibits growth and induces cell death in hepatoma HepG2 cells

Nor Ashikeen Mukti, Mariati Abdul Rahman, Saiful Anuar Karsani, Wan Zurinah Wan Ngah, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

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Abstract

Objective: Studies have shown that Chlorella vulgaris (ChV), a unicellular green microalgae, exhibited diverse health beneficial effects in animal and human studies, including antitumour activity. It is believed from previous studies that a glycoprotein is responsible for the antitumor effect. The aim of our study was to purify the glycoprotein secreted by a Malaysia strain of the ChV into its culture medium, i.e. Bold's Basal Medium (BBM), and to investigate its antitumour properties. Design: Fast Protein Liquid Chromatography (FPLC) was utilised to isolate the protein of interest by using anion exchange chromatography. Mass-spectrometry was employed to identify the novel protein. Four protein fractions were eluted and subjected to SDS-PAGE and Silver staining. The protein bands from all four fractions appeared to have similar molecular weight at approximately 70 kDa and they were pooled for the study of antitumour activity. Results: Viability assay showed that the protein has an IC50 of 1.75 μg/ml against HepG2 cells. At similar concentration, it also scored the highest apoptotic activity, measured by DNA fragmentation unit of 8.72. However, Western Blot analysis revealed that neither the intrinsic nor the extrinsic pathways could be attributed to the apoptotic effect of the protein fraction, but the antiapoptotic protein BCL-2 was found to be raised. Overexpression of BCL-2 could possibly resulted in the inhibition of extrinsic mediated apoptosis. Conclusion: We hypothesized that the induction of cell death by the novel protein fraction purified from the culture media of Chlorella vulgaris was attributed to another mode of cell death other than apoptosis.

Original languageEnglish
Pages (from-to)140-145
Number of pages6
JournalInternational Medical Journal
Volume20
Issue number2
Publication statusPublished - Apr 2013

Fingerprint

Chlorella vulgaris
Hep G2 Cells
Culture Media
Hepatocellular Carcinoma
Cell Death
Growth
Proteins
Glycoproteins
Apoptosis
Microalgae
Silver Staining
Malaysia
DNA Fragmentation
Liquid Chromatography
Inhibitory Concentration 50
Anions
Chromatography
Polyacrylamide Gel Electrophoresis
Mass Spectrometry
Molecular Weight

Keywords

  • Antitumour
  • Apoptosis
  • Chlorella vulgaris
  • Glycoprotein
  • HepG2 cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A purified novel protein from the culture media of Chlorella vulgaris inhibits growth and induces cell death in hepatoma HepG2 cells. / Mukti, Nor Ashikeen; Abdul Rahman, Mariati; Karsani, Saiful Anuar; Wan Ngah, Wan Zurinah; Mohd Yusof, Yasmin Anum.

In: International Medical Journal, Vol. 20, No. 2, 04.2013, p. 140-145.

Research output: Contribution to journalArticle

Mukti, Nor Ashikeen ; Abdul Rahman, Mariati ; Karsani, Saiful Anuar ; Wan Ngah, Wan Zurinah ; Mohd Yusof, Yasmin Anum. / A purified novel protein from the culture media of Chlorella vulgaris inhibits growth and induces cell death in hepatoma HepG2 cells. In: International Medical Journal. 2013 ; Vol. 20, No. 2. pp. 140-145.
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