A prospective cohort study on IRS gene polymorphisms in type 2 diabetes mellitus patients during severe/acute hyperglycemia phase 1: Association with insulin resistance

Hasniza Zaman Huri, Mohd Makmor Bakry, Rosnani Hashim, Norlaila Mustafa, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

Abstract

Purpose: To investigate the genetic polymorphisms that may contribute to the worsening of insulin resistance in type 2 diabetes mellitus (T2DM) with severe or acute hyperglycemia. Methods: This is a prospective cohort study involving 156 T2DM patients with severe or acute hyperglycemia from all medical wards of the National University of Malaysia Medical Centre (UKMMC) that were placed on insulin therapy. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the genetic association of insulin receptor substrate (IRS) gene with insulin resistance. Insulin resistance status was determined using the homeostatic model assessment for insulin resistance (HOMA-IR) index. Results: IRS1 polymorphisms were associated with increased insulin resistance (X2= 5.09, p = 0.023) in T2DM patients with severe/acute hyperglycemia. IRS2 polymorphisms were not associated with insulin resistance (X2 = 0.69, p = 0.406) in this group of patients. Conclusion: IRS1 genetic factor alone may be a significant genetic determinant for insulin resistance in T2DM patients during severe/acute phase hyperglycemia.

Original languageEnglish
Pages (from-to)895-901
Number of pages7
JournalTropical Journal of Pharmaceutical Research
Volume13
Issue number6
DOIs
Publication statusPublished - 2014

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Insulin Receptor
Hyperglycemia
Type 2 Diabetes Mellitus
Insulin Resistance
Cohort Studies
Prospective Studies
Genes
Malaysia
Genetic Polymorphisms
Restriction Fragment Length Polymorphisms
Insulin
Polymerase Chain Reaction

Keywords

  • Diabetes
  • Genetic
  • Hyperglycemia
  • Insulin receptor substrate
  • Insulin resistance
  • IRS1
  • IRS2
  • Polymorphism

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

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title = "A prospective cohort study on IRS gene polymorphisms in type 2 diabetes mellitus patients during severe/acute hyperglycemia phase 1: Association with insulin resistance",
abstract = "Purpose: To investigate the genetic polymorphisms that may contribute to the worsening of insulin resistance in type 2 diabetes mellitus (T2DM) with severe or acute hyperglycemia. Methods: This is a prospective cohort study involving 156 T2DM patients with severe or acute hyperglycemia from all medical wards of the National University of Malaysia Medical Centre (UKMMC) that were placed on insulin therapy. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the genetic association of insulin receptor substrate (IRS) gene with insulin resistance. Insulin resistance status was determined using the homeostatic model assessment for insulin resistance (HOMA-IR) index. Results: IRS1 polymorphisms were associated with increased insulin resistance (X2= 5.09, p = 0.023) in T2DM patients with severe/acute hyperglycemia. IRS2 polymorphisms were not associated with insulin resistance (X2 = 0.69, p = 0.406) in this group of patients. Conclusion: IRS1 genetic factor alone may be a significant genetic determinant for insulin resistance in T2DM patients during severe/acute phase hyperglycemia.",
keywords = "Diabetes, Genetic, Hyperglycemia, Insulin receptor substrate, Insulin resistance, IRS1, IRS2, Polymorphism",
author = "Huri, {Hasniza Zaman} and {Makmor Bakry}, Mohd and Rosnani Hashim and Norlaila Mustafa and {Wan Ngah}, {Wan Zurinah}",
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T2 - Association with insulin resistance

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AU - Makmor Bakry, Mohd

AU - Hashim, Rosnani

AU - Mustafa, Norlaila

AU - Wan Ngah, Wan Zurinah

PY - 2014

Y1 - 2014

N2 - Purpose: To investigate the genetic polymorphisms that may contribute to the worsening of insulin resistance in type 2 diabetes mellitus (T2DM) with severe or acute hyperglycemia. Methods: This is a prospective cohort study involving 156 T2DM patients with severe or acute hyperglycemia from all medical wards of the National University of Malaysia Medical Centre (UKMMC) that were placed on insulin therapy. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the genetic association of insulin receptor substrate (IRS) gene with insulin resistance. Insulin resistance status was determined using the homeostatic model assessment for insulin resistance (HOMA-IR) index. Results: IRS1 polymorphisms were associated with increased insulin resistance (X2= 5.09, p = 0.023) in T2DM patients with severe/acute hyperglycemia. IRS2 polymorphisms were not associated with insulin resistance (X2 = 0.69, p = 0.406) in this group of patients. Conclusion: IRS1 genetic factor alone may be a significant genetic determinant for insulin resistance in T2DM patients during severe/acute phase hyperglycemia.

AB - Purpose: To investigate the genetic polymorphisms that may contribute to the worsening of insulin resistance in type 2 diabetes mellitus (T2DM) with severe or acute hyperglycemia. Methods: This is a prospective cohort study involving 156 T2DM patients with severe or acute hyperglycemia from all medical wards of the National University of Malaysia Medical Centre (UKMMC) that were placed on insulin therapy. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the genetic association of insulin receptor substrate (IRS) gene with insulin resistance. Insulin resistance status was determined using the homeostatic model assessment for insulin resistance (HOMA-IR) index. Results: IRS1 polymorphisms were associated with increased insulin resistance (X2= 5.09, p = 0.023) in T2DM patients with severe/acute hyperglycemia. IRS2 polymorphisms were not associated with insulin resistance (X2 = 0.69, p = 0.406) in this group of patients. Conclusion: IRS1 genetic factor alone may be a significant genetic determinant for insulin resistance in T2DM patients during severe/acute phase hyperglycemia.

KW - Diabetes

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KW - Insulin resistance

KW - IRS1

KW - IRS2

KW - Polymorphism

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