A Preliminary Study on Coagulation Parameters and Sterility of Thawed Refrozen Fresh Frozen Plasma

Rahayu Draman, Rabeya Yousuf, Suria Abdul Aziz, Chuan Hun Ding, Suzana Zainol, Leong Chooi Fun

Research output: Contribution to journalArticle

Abstract

Thawed fresh frozen plasma (FFP) if not used within 6 h, may have to be discarded due to the risk of contamination and uncertainty about its quality. The main objective of this study was to evaluate the levels of coagulation Factor II (FII), Factor VIII (FVIII), fibrinogen and bacterial growth in thawed refrozen FFP. Thirty FFP samples were collected from healthy donors. FFP were thawed in water bath at 37 °C for 20–25 min. Approximately 10 mL of plasma from each FFP unit was tested for FII, FVIII, fibrinogen and sterility. The thawed FFP units were then kept at 4 °C for 6 h before being refrozen and stored at − 20 °C. Two weeks later, the refrozen FFP were thawed again and representative samples were analysed as before. There was a significant decline in the mean FVIII level, from 155.77% to 85.6% at second thaw. The mean FII level increased significantly from 74.9% to 82%, whereas the mean fibrinogen level fell from 3.34g/L to 3.28 g/L, but the decline was not statistically significant. There was no bacterial contamination in all samples at both time points. Refrozen plasma may be considered as an alternative to the storage of thawed unused FFP provided they are kept in a controlled environment to reduce wastage. These thawed refrozen FFP can be used later in bleeding cases like other FFP as the levels of FVIII are still within the standard haematology range (0.5–2 IU/mL) and above the minimal level of 30% coagulation factors required for adequate haemostasis.

Original languageEnglish
JournalIndian Journal of Hematology and Blood Transfusion
DOIs
Publication statusAccepted/In press - 1 Jan 2019

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Infertility
Factor VIII
Prothrombin
Fibrinogen
Controlled Environment
Blood Coagulation Factors
Hematology
Hemostasis
Baths
Uncertainty
Hemorrhage
Water

Keywords

  • Coagulation parameters
  • Refrozen
  • Sterility
  • Thawed fresh frozen plasma

ASJC Scopus subject areas

  • Hematology

Cite this

@article{cfc2ac1db1f943a5b44ae5e1b2c1ec9f,
title = "A Preliminary Study on Coagulation Parameters and Sterility of Thawed Refrozen Fresh Frozen Plasma",
abstract = "Thawed fresh frozen plasma (FFP) if not used within 6 h, may have to be discarded due to the risk of contamination and uncertainty about its quality. The main objective of this study was to evaluate the levels of coagulation Factor II (FII), Factor VIII (FVIII), fibrinogen and bacterial growth in thawed refrozen FFP. Thirty FFP samples were collected from healthy donors. FFP were thawed in water bath at 37 °C for 20–25 min. Approximately 10 mL of plasma from each FFP unit was tested for FII, FVIII, fibrinogen and sterility. The thawed FFP units were then kept at 4 °C for 6 h before being refrozen and stored at − 20 °C. Two weeks later, the refrozen FFP were thawed again and representative samples were analysed as before. There was a significant decline in the mean FVIII level, from 155.77{\%} to 85.6{\%} at second thaw. The mean FII level increased significantly from 74.9{\%} to 82{\%}, whereas the mean fibrinogen level fell from 3.34g/L to 3.28 g/L, but the decline was not statistically significant. There was no bacterial contamination in all samples at both time points. Refrozen plasma may be considered as an alternative to the storage of thawed unused FFP provided they are kept in a controlled environment to reduce wastage. These thawed refrozen FFP can be used later in bleeding cases like other FFP as the levels of FVIII are still within the standard haematology range (0.5–2 IU/mL) and above the minimal level of 30{\%} coagulation factors required for adequate haemostasis.",
keywords = "Coagulation parameters, Refrozen, Sterility, Thawed fresh frozen plasma",
author = "Rahayu Draman and Rabeya Yousuf and {Abdul Aziz}, Suria and Ding, {Chuan Hun} and Suzana Zainol and {Chooi Fun}, Leong",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s12288-019-01171-0",
language = "English",
journal = "Indian Journal of Hematology and Blood Transfusion",
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T1 - A Preliminary Study on Coagulation Parameters and Sterility of Thawed Refrozen Fresh Frozen Plasma

AU - Draman, Rahayu

AU - Yousuf, Rabeya

AU - Abdul Aziz, Suria

AU - Ding, Chuan Hun

AU - Zainol, Suzana

AU - Chooi Fun, Leong

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Thawed fresh frozen plasma (FFP) if not used within 6 h, may have to be discarded due to the risk of contamination and uncertainty about its quality. The main objective of this study was to evaluate the levels of coagulation Factor II (FII), Factor VIII (FVIII), fibrinogen and bacterial growth in thawed refrozen FFP. Thirty FFP samples were collected from healthy donors. FFP were thawed in water bath at 37 °C for 20–25 min. Approximately 10 mL of plasma from each FFP unit was tested for FII, FVIII, fibrinogen and sterility. The thawed FFP units were then kept at 4 °C for 6 h before being refrozen and stored at − 20 °C. Two weeks later, the refrozen FFP were thawed again and representative samples were analysed as before. There was a significant decline in the mean FVIII level, from 155.77% to 85.6% at second thaw. The mean FII level increased significantly from 74.9% to 82%, whereas the mean fibrinogen level fell from 3.34g/L to 3.28 g/L, but the decline was not statistically significant. There was no bacterial contamination in all samples at both time points. Refrozen plasma may be considered as an alternative to the storage of thawed unused FFP provided they are kept in a controlled environment to reduce wastage. These thawed refrozen FFP can be used later in bleeding cases like other FFP as the levels of FVIII are still within the standard haematology range (0.5–2 IU/mL) and above the minimal level of 30% coagulation factors required for adequate haemostasis.

AB - Thawed fresh frozen plasma (FFP) if not used within 6 h, may have to be discarded due to the risk of contamination and uncertainty about its quality. The main objective of this study was to evaluate the levels of coagulation Factor II (FII), Factor VIII (FVIII), fibrinogen and bacterial growth in thawed refrozen FFP. Thirty FFP samples were collected from healthy donors. FFP were thawed in water bath at 37 °C for 20–25 min. Approximately 10 mL of plasma from each FFP unit was tested for FII, FVIII, fibrinogen and sterility. The thawed FFP units were then kept at 4 °C for 6 h before being refrozen and stored at − 20 °C. Two weeks later, the refrozen FFP were thawed again and representative samples were analysed as before. There was a significant decline in the mean FVIII level, from 155.77% to 85.6% at second thaw. The mean FII level increased significantly from 74.9% to 82%, whereas the mean fibrinogen level fell from 3.34g/L to 3.28 g/L, but the decline was not statistically significant. There was no bacterial contamination in all samples at both time points. Refrozen plasma may be considered as an alternative to the storage of thawed unused FFP provided they are kept in a controlled environment to reduce wastage. These thawed refrozen FFP can be used later in bleeding cases like other FFP as the levels of FVIII are still within the standard haematology range (0.5–2 IU/mL) and above the minimal level of 30% coagulation factors required for adequate haemostasis.

KW - Coagulation parameters

KW - Refrozen

KW - Sterility

KW - Thawed fresh frozen plasma

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