A Preliminary study of rotenone exhaustive extraction kinetic from Derris elliptica dried roots using normal soaking extraction (NSE) method

Saiful Irwan Zubairi, Mohamad Roji Sarmidi, Ramlan Abdul Aziz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Currently bio-pesticide is relatively harmless to humans and the environment and thus desirable for the use in the controlling of insect vectors. The importance of bio-pesticide in both scale commercial agriculture and small plot, subsistence farming has been on the rise. One of the sources of bio-pesticide is 'tuba' plant, also known as Derris elliptica. Derris elliptica contains bio-active compound known as rotenone (C23H33O6) which is harmless to plants, highly toxic to many insects and relatively innocuous to mammals. The study carried out was to investigate the appropriate independent and dependent variables of the exhaustive extraction process by evaluating the kinetic equilibrium phase of the normal soaking extraction (NSE) method. The rotenone from the roots and stems was extracted by using the normal soaking extraction (NSE) at an ambient temperature of 28 to 30 °C with 95% (v/v) of acetone as a solvent. The solvent-to-solid ratio of the extraction was 10 ml/g. The extraction was carried out for 1440 mins and the fractions of the liquid crude extract were collected for each interval time (30 mins/ml/fraction) and were further cleaned up to remove any fine debris of roots and stems prior to the determination of rotenone content (mg) and its concentration (mg/ml) by using the reverse-phase high performance liquid chromatography (RP-HPLC). Based on the results obtained, it was found that the best extraction time was between 800 mins to 840 mins with the yield of rotenone and rotenone concentration of 830 mg to 890 mg and 2800 ppm to 2950 ppm respectively. Thus, the identified exhaustive extraction parameters can be utilized to optimize the yield of rotenone and its concentration via statistical analysis.

Original languageEnglish
Pages (from-to)910-915
Number of pages6
JournalAdvances in Environmental Biology
Volume8
Issue number4
Publication statusPublished - 2014

Fingerprint

Derris elliptica
Derris
Rotenone
rotenone
extraction method
soaking
kinetics
Pesticides
pesticide
pesticides
Agriculture
methodology
stem
Toxic Plants
Insect Vectors
insect
subsistence farming
phase equilibrium
Reverse-Phase Chromatography
insect vectors

Keywords

  • Derris elliptica
  • Exhaustive extraction
  • Liquid crude extract
  • Normal soaking extraction
  • Rotenone

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

A Preliminary study of rotenone exhaustive extraction kinetic from Derris elliptica dried roots using normal soaking extraction (NSE) method. / Zubairi, Saiful Irwan; Sarmidi, Mohamad Roji; Aziz, Ramlan Abdul.

In: Advances in Environmental Biology, Vol. 8, No. 4, 2014, p. 910-915.

Research output: Contribution to journalArticle

@article{1c0166a185e1452da4d9bf30897ed984,
title = "A Preliminary study of rotenone exhaustive extraction kinetic from Derris elliptica dried roots using normal soaking extraction (NSE) method",
abstract = "Currently bio-pesticide is relatively harmless to humans and the environment and thus desirable for the use in the controlling of insect vectors. The importance of bio-pesticide in both scale commercial agriculture and small plot, subsistence farming has been on the rise. One of the sources of bio-pesticide is 'tuba' plant, also known as Derris elliptica. Derris elliptica contains bio-active compound known as rotenone (C23H33O6) which is harmless to plants, highly toxic to many insects and relatively innocuous to mammals. The study carried out was to investigate the appropriate independent and dependent variables of the exhaustive extraction process by evaluating the kinetic equilibrium phase of the normal soaking extraction (NSE) method. The rotenone from the roots and stems was extracted by using the normal soaking extraction (NSE) at an ambient temperature of 28 to 30 °C with 95{\%} (v/v) of acetone as a solvent. The solvent-to-solid ratio of the extraction was 10 ml/g. The extraction was carried out for 1440 mins and the fractions of the liquid crude extract were collected for each interval time (30 mins/ml/fraction) and were further cleaned up to remove any fine debris of roots and stems prior to the determination of rotenone content (mg) and its concentration (mg/ml) by using the reverse-phase high performance liquid chromatography (RP-HPLC). Based on the results obtained, it was found that the best extraction time was between 800 mins to 840 mins with the yield of rotenone and rotenone concentration of 830 mg to 890 mg and 2800 ppm to 2950 ppm respectively. Thus, the identified exhaustive extraction parameters can be utilized to optimize the yield of rotenone and its concentration via statistical analysis.",
keywords = "Derris elliptica, Exhaustive extraction, Liquid crude extract, Normal soaking extraction, Rotenone",
author = "Zubairi, {Saiful Irwan} and Sarmidi, {Mohamad Roji} and Aziz, {Ramlan Abdul}",
year = "2014",
language = "English",
volume = "8",
pages = "910--915",
journal = "Advances in Environmental Biology",
issn = "1995-0756",
publisher = "American-Eurasian Network for Scientific Information",
number = "4",

}

TY - JOUR

T1 - A Preliminary study of rotenone exhaustive extraction kinetic from Derris elliptica dried roots using normal soaking extraction (NSE) method

AU - Zubairi, Saiful Irwan

AU - Sarmidi, Mohamad Roji

AU - Aziz, Ramlan Abdul

PY - 2014

Y1 - 2014

N2 - Currently bio-pesticide is relatively harmless to humans and the environment and thus desirable for the use in the controlling of insect vectors. The importance of bio-pesticide in both scale commercial agriculture and small plot, subsistence farming has been on the rise. One of the sources of bio-pesticide is 'tuba' plant, also known as Derris elliptica. Derris elliptica contains bio-active compound known as rotenone (C23H33O6) which is harmless to plants, highly toxic to many insects and relatively innocuous to mammals. The study carried out was to investigate the appropriate independent and dependent variables of the exhaustive extraction process by evaluating the kinetic equilibrium phase of the normal soaking extraction (NSE) method. The rotenone from the roots and stems was extracted by using the normal soaking extraction (NSE) at an ambient temperature of 28 to 30 °C with 95% (v/v) of acetone as a solvent. The solvent-to-solid ratio of the extraction was 10 ml/g. The extraction was carried out for 1440 mins and the fractions of the liquid crude extract were collected for each interval time (30 mins/ml/fraction) and were further cleaned up to remove any fine debris of roots and stems prior to the determination of rotenone content (mg) and its concentration (mg/ml) by using the reverse-phase high performance liquid chromatography (RP-HPLC). Based on the results obtained, it was found that the best extraction time was between 800 mins to 840 mins with the yield of rotenone and rotenone concentration of 830 mg to 890 mg and 2800 ppm to 2950 ppm respectively. Thus, the identified exhaustive extraction parameters can be utilized to optimize the yield of rotenone and its concentration via statistical analysis.

AB - Currently bio-pesticide is relatively harmless to humans and the environment and thus desirable for the use in the controlling of insect vectors. The importance of bio-pesticide in both scale commercial agriculture and small plot, subsistence farming has been on the rise. One of the sources of bio-pesticide is 'tuba' plant, also known as Derris elliptica. Derris elliptica contains bio-active compound known as rotenone (C23H33O6) which is harmless to plants, highly toxic to many insects and relatively innocuous to mammals. The study carried out was to investigate the appropriate independent and dependent variables of the exhaustive extraction process by evaluating the kinetic equilibrium phase of the normal soaking extraction (NSE) method. The rotenone from the roots and stems was extracted by using the normal soaking extraction (NSE) at an ambient temperature of 28 to 30 °C with 95% (v/v) of acetone as a solvent. The solvent-to-solid ratio of the extraction was 10 ml/g. The extraction was carried out for 1440 mins and the fractions of the liquid crude extract were collected for each interval time (30 mins/ml/fraction) and were further cleaned up to remove any fine debris of roots and stems prior to the determination of rotenone content (mg) and its concentration (mg/ml) by using the reverse-phase high performance liquid chromatography (RP-HPLC). Based on the results obtained, it was found that the best extraction time was between 800 mins to 840 mins with the yield of rotenone and rotenone concentration of 830 mg to 890 mg and 2800 ppm to 2950 ppm respectively. Thus, the identified exhaustive extraction parameters can be utilized to optimize the yield of rotenone and its concentration via statistical analysis.

KW - Derris elliptica

KW - Exhaustive extraction

KW - Liquid crude extract

KW - Normal soaking extraction

KW - Rotenone

UR - http://www.scopus.com/inward/record.url?scp=84899854103&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899854103&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84899854103

VL - 8

SP - 910

EP - 915

JO - Advances in Environmental Biology

JF - Advances in Environmental Biology

SN - 1995-0756

IS - 4

ER -