A population study of the normal cornea using an in Vivo, slit-scanning confocal microscope

Jo Hollingsworth, Inma Perez-Gomez, Haliza Abdul Mutalib, Nathan Efron

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Purpose. To document qualitative and quantitative changes in the normal, healthy human cornea with age using the confocal microscope. Methods. The central corneas of 120 subjects (mean age, 41 years; range, 11 to 80 years) were examined using an in vivo slit-scanning real-time confocal microscope. Images of the corneal stroma and endothelium from both eyes of each subject were semiautomatically analyzed in an observer-masked, randomized manner. Results. Anterior keratocyte density, posterior keratocyte density, and endothelial cell density were shown to be unaffected by the sex of the subject with p values of 0.46, 0.55, 0.50, respectively (multivariate analysis of variance). No statistically significant difference was detected between right and left eyes for all corneal layers examined. The anterior keratocyte density, posterior keratocyte density, and endothelial cell density decreased at a rate of 0.48, 0.22, and 0.33% per year, respectively. A positive correlation was found between the coefficient of cell variation and age. Conclusions. This data constitutes essential normative data that can be used as a control in further research into abnormal corneal conditions.

Original languageEnglish
Pages (from-to)706-711
Number of pages6
JournalOptometry and Vision Science
Volume78
Issue number10
Publication statusPublished - 2001
Externally publishedYes

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Cornea
Endothelial Cells
Cell Count
Corneal Stroma
Corneal Endothelium
Population
Analysis of Variance
Multivariate Analysis
Research

Keywords

  • Confocal microscopy
  • Endothelial cell density
  • Keratocyte density
  • Normal cornea

ASJC Scopus subject areas

  • Ophthalmology

Cite this

A population study of the normal cornea using an in Vivo, slit-scanning confocal microscope. / Hollingsworth, Jo; Perez-Gomez, Inma; Abdul Mutalib, Haliza; Efron, Nathan.

In: Optometry and Vision Science, Vol. 78, No. 10, 2001, p. 706-711.

Research output: Contribution to journalArticle

Hollingsworth, Jo ; Perez-Gomez, Inma ; Abdul Mutalib, Haliza ; Efron, Nathan. / A population study of the normal cornea using an in Vivo, slit-scanning confocal microscope. In: Optometry and Vision Science. 2001 ; Vol. 78, No. 10. pp. 706-711.
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