A plant transformation vector containing the gene dehd for the development of cultivars resistant to monochloroacetic acid

E. Mohamed, F. A. Rahiman, R. A. Wahab, Che Radziah Che Mohd. Zain, M. A. Javed, F. Huyop

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A dehalogenase D gene (dehD) capable of degrading monochloroacetic acid (MCA) has been previously isolated from Rhizobium sp. RC1 and characterized. The 804-bp dehD gene was inserted into pCAMBIA under the control of the cauliflower mosaic virus 35S promoter and designated pCAMdehD, with a total size of 10,592 bp. The pCAMdehD was introduced into tobacco (Nicotiana benthamiana) via Agrobacterium-mediated transformation. The integration and expression of dehD in N. benthamiana was confirmed by PCR and reverse transcription PCR, respectively. MCA-resistant transformants were selected in tissue cultures containing 60 µg/L MCA. Analysis of plants using a leaf-painting assay revealed that transgenic N. benthamiana was resistant to 4.0 g/L MCA compared with 2.0 g/L for non-transformed controls. The use of dehD could thus be advantageous for herbicide-resistant plant breeding systems, and it is also a suitable marker gene for plant-transformation studies. To the best of our knowledge, this is the first report detailing transgenic N. benthamiana engineered with dehD from Rhizobium sp. RC1.

Original languageEnglish
Pages (from-to)1133-1139
Number of pages7
JournalJournal of Animal and Plant Sciences
Volume26
Issue number4
Publication statusPublished - 1 Aug 2016

Fingerprint

monochloroacetic acid
Nicotiana benthamiana
cultivars
genes
Rhizobium
genetically modified organisms
Cauliflower mosaic virus
plant breeding
Agrobacterium
tissue culture
tobacco
herbicides
reverse transcriptase polymerase chain reaction
promoter regions
genetic markers
assays

Keywords

  • Binary vector
  • DehD gene
  • Herbicide resistant
  • Monochloroacetic acid
  • Tobacco

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Plant Science

Cite this

A plant transformation vector containing the gene dehd for the development of cultivars resistant to monochloroacetic acid. / Mohamed, E.; Rahiman, F. A.; Wahab, R. A.; Che Mohd. Zain, Che Radziah; Javed, M. A.; Huyop, F.

In: Journal of Animal and Plant Sciences, Vol. 26, No. 4, 01.08.2016, p. 1133-1139.

Research output: Contribution to journalArticle

@article{94a7e422f02b4a5a9063bdd2a81f8752,
title = "A plant transformation vector containing the gene dehd for the development of cultivars resistant to monochloroacetic acid",
abstract = "A dehalogenase D gene (dehD) capable of degrading monochloroacetic acid (MCA) has been previously isolated from Rhizobium sp. RC1 and characterized. The 804-bp dehD gene was inserted into pCAMBIA under the control of the cauliflower mosaic virus 35S promoter and designated pCAMdehD, with a total size of 10,592 bp. The pCAMdehD was introduced into tobacco (Nicotiana benthamiana) via Agrobacterium-mediated transformation. The integration and expression of dehD in N. benthamiana was confirmed by PCR and reverse transcription PCR, respectively. MCA-resistant transformants were selected in tissue cultures containing 60 µg/L MCA. Analysis of plants using a leaf-painting assay revealed that transgenic N. benthamiana was resistant to 4.0 g/L MCA compared with 2.0 g/L for non-transformed controls. The use of dehD could thus be advantageous for herbicide-resistant plant breeding systems, and it is also a suitable marker gene for plant-transformation studies. To the best of our knowledge, this is the first report detailing transgenic N. benthamiana engineered with dehD from Rhizobium sp. RC1.",
keywords = "Binary vector, DehD gene, Herbicide resistant, Monochloroacetic acid, Tobacco",
author = "E. Mohamed and Rahiman, {F. A.} and Wahab, {R. A.} and {Che Mohd. Zain}, {Che Radziah} and Javed, {M. A.} and F. Huyop",
year = "2016",
month = "8",
day = "1",
language = "English",
volume = "26",
pages = "1133--1139",
journal = "Journal of Animal and Plant Sciences",
issn = "1018-7081",
publisher = "Pakistan Agricultural Scientists Forum",
number = "4",

}

TY - JOUR

T1 - A plant transformation vector containing the gene dehd for the development of cultivars resistant to monochloroacetic acid

AU - Mohamed, E.

AU - Rahiman, F. A.

AU - Wahab, R. A.

AU - Che Mohd. Zain, Che Radziah

AU - Javed, M. A.

AU - Huyop, F.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - A dehalogenase D gene (dehD) capable of degrading monochloroacetic acid (MCA) has been previously isolated from Rhizobium sp. RC1 and characterized. The 804-bp dehD gene was inserted into pCAMBIA under the control of the cauliflower mosaic virus 35S promoter and designated pCAMdehD, with a total size of 10,592 bp. The pCAMdehD was introduced into tobacco (Nicotiana benthamiana) via Agrobacterium-mediated transformation. The integration and expression of dehD in N. benthamiana was confirmed by PCR and reverse transcription PCR, respectively. MCA-resistant transformants were selected in tissue cultures containing 60 µg/L MCA. Analysis of plants using a leaf-painting assay revealed that transgenic N. benthamiana was resistant to 4.0 g/L MCA compared with 2.0 g/L for non-transformed controls. The use of dehD could thus be advantageous for herbicide-resistant plant breeding systems, and it is also a suitable marker gene for plant-transformation studies. To the best of our knowledge, this is the first report detailing transgenic N. benthamiana engineered with dehD from Rhizobium sp. RC1.

AB - A dehalogenase D gene (dehD) capable of degrading monochloroacetic acid (MCA) has been previously isolated from Rhizobium sp. RC1 and characterized. The 804-bp dehD gene was inserted into pCAMBIA under the control of the cauliflower mosaic virus 35S promoter and designated pCAMdehD, with a total size of 10,592 bp. The pCAMdehD was introduced into tobacco (Nicotiana benthamiana) via Agrobacterium-mediated transformation. The integration and expression of dehD in N. benthamiana was confirmed by PCR and reverse transcription PCR, respectively. MCA-resistant transformants were selected in tissue cultures containing 60 µg/L MCA. Analysis of plants using a leaf-painting assay revealed that transgenic N. benthamiana was resistant to 4.0 g/L MCA compared with 2.0 g/L for non-transformed controls. The use of dehD could thus be advantageous for herbicide-resistant plant breeding systems, and it is also a suitable marker gene for plant-transformation studies. To the best of our knowledge, this is the first report detailing transgenic N. benthamiana engineered with dehD from Rhizobium sp. RC1.

KW - Binary vector

KW - DehD gene

KW - Herbicide resistant

KW - Monochloroacetic acid

KW - Tobacco

UR - http://www.scopus.com/inward/record.url?scp=84984908570&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84984908570&partnerID=8YFLogxK

M3 - Article

VL - 26

SP - 1133

EP - 1139

JO - Journal of Animal and Plant Sciences

JF - Journal of Animal and Plant Sciences

SN - 1018-7081

IS - 4

ER -