A pilot study on cytotoxic T lymphocyte-4 gene polymorphisms in urinary schistosomiasis

Zulkarnain Md Idris, Maria Yazdanbakhsh, Ayola Akim Adegnika, Bertrand Lell, Saadou Issifou, Rahmah Noordin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Urinary schistosomiasis is caused by the digenetic trematode Schistosoma haematobium, characterized by accumulation of eggs in the genitourinary tract. Cytotoxic T-lymphocyte antigen 4 (CTLA-4) can play an important role in parasitic infection due to its major role as a negative regulator of T-cell activation and proliferation. This study was performed in patients with schistosomiasis and healthy controls to analyze the allele and genotype frequencies of four CTLA-4 gene polymorphisms. The CTLA-4 gene was amplified using Taqman real-time polymerase chain reaction, and allele and genotypes of 49 patients with schistosomiasis were analyzed using allelic discrimination analysis followed by subsequent direct sequencing. The results were compared with healthy control subjects. The frequencies of CTLA-4 rs733618 A allele at position -1722 (p=0.001), rs11571316 C allele at position -1577 (p<0.001), and rs231775 A allele at position +49 (p=0.002) in the patient group were significantly higher than the control group. The rs733618 AA genotype (p=0.001), rs11571316 CC genotype (p<0.001), and rs231775 AA genotype (p=0.007) were also significantly overrepresented. Meanwhile, rs733618 AG genotype (p=0.001), rs11571316 CT genotype (p=0.02), and rs231775 GG genotype (p=0.029) were significantly decreased in the patients with schistosomiasis, as compared with the controls. No significant difference was observed in both allele and genotype of rs16841252. The results of this study suggest that the rs733618, rs11571316, and rs231775 polymorphisms in the CTLA-4 gene may influence susceptibility to schistosomiasis infection in the Gabonese children.

Original languageEnglish
Pages (from-to)488-492
Number of pages5
JournalGenetic Testing and Molecular Biomarkers
Volume16
Issue number6
DOIs
Publication statusPublished - 1 Jun 2012
Externally publishedYes

Fingerprint

Schistosomiasis haematobia
Cytotoxic T-Lymphocytes
CTLA-4 Antigen
Genotype
Schistosomiasis
Genes
Alleles
Schistosoma haematobium
Parasitic Diseases
Gene Frequency
Eggs
Real-Time Polymerase Chain Reaction
Healthy Volunteers
Cell Proliferation

ASJC Scopus subject areas

  • Genetics(clinical)

Cite this

A pilot study on cytotoxic T lymphocyte-4 gene polymorphisms in urinary schistosomiasis. / Md Idris, Zulkarnain; Yazdanbakhsh, Maria; Adegnika, Ayola Akim; Lell, Bertrand; Issifou, Saadou; Noordin, Rahmah.

In: Genetic Testing and Molecular Biomarkers, Vol. 16, No. 6, 01.06.2012, p. 488-492.

Research output: Contribution to journalArticle

Md Idris, Zulkarnain ; Yazdanbakhsh, Maria ; Adegnika, Ayola Akim ; Lell, Bertrand ; Issifou, Saadou ; Noordin, Rahmah. / A pilot study on cytotoxic T lymphocyte-4 gene polymorphisms in urinary schistosomiasis. In: Genetic Testing and Molecular Biomarkers. 2012 ; Vol. 16, No. 6. pp. 488-492.
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