A pillar-based microfilter for isolation of white blood cells on elastomeric substrate

Jafar Alvankarian, Alireza Bahadorimehr, Burhanuddin Yeop Majlis

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Our goal is to design, fabricate, and characterize a pillar-based microfluidic device for size-based separation of human blood cells on an elastomeric substrate with application in the low-cost rapid prototyping of lab-chip devices. The single inlet single outlet device is using parallel U-shape arrays of pillars with cutoff size of 5.5 μm for trapping white blood cells (WBCs) in a pillar chamber with internal dead-volume of less than 1.0 μl. The microstructures are designed to limit the elastomeric deformation against fluid pressures. Numerical analysis showed that at maximum pressure loss of 15 kPa which is lower than the device conformal bonding strength, the pillar elastomeric deformation is less than 5% for flow rates of up to 1.0 ml min-1. Molding technique was employed for device prototyping using polyurethane methacrylate (PUMA) resin and polydimethylsiloxane (PDMS) mold. Characterization of the dual-layer device with beads and blood samples is performed. Tests with blood injection showed that ~18%-25% of WBCs are trapped and ~84%-89% of red blood cells (RBCs) are passed at flow rates of 15-50 μl min-1 with a slight decrease of WBCs trap and improve of the RBCs pass at higher flow rates. Similar results were obtained by separation of mixed microspheres of different size injected at flow rates of up to 400 μl min-1. Tests with blood samples stained by fluorescent gel demonstrated that the WBCs are accumulated in the arrays of pillars that later end up to blockage of the device. Filtration results of using elastomeric substrate present a good consistency with the trend of separation efficiencies of the similar silicon-based filters.

Original languageEnglish
Article number014102
JournalBiomicrofluidics
Volume7
Issue number1
DOIs
Publication statusPublished - 4 Feb 2013

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leukocytes
isolation
Leukocytes
Blood
Cells
Equipment and Supplies
Substrates
flow velocity
blood
erythrocytes
Hematologic Tests
Lab-On-A-Chip Devices
Flow rate
polyurethane resins
Erythrocytes
Pressure
rapid prototyping
blood cells
acrylic resins
Polyurethanes

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Condensed Matter Physics
  • Materials Science(all)
  • Physical and Theoretical Chemistry

Cite this

A pillar-based microfilter for isolation of white blood cells on elastomeric substrate. / Alvankarian, Jafar; Bahadorimehr, Alireza; Yeop Majlis, Burhanuddin.

In: Biomicrofluidics, Vol. 7, No. 1, 014102, 04.02.2013.

Research output: Contribution to journalArticle

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