A PCR-based approach to sequence the Candida tropicalis HSP90 gene

Jacinta Santhanam, James P. Burnie

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The heat shock protein 90 (hsp90) gene sequence is known to be highly conserved across the species barrier. A PCR-based method was thus utilised in an attempt to sequence the Candida tropicalis hsp90 gene. Primers for PCR were designed from conserved regions of the gene, which were identified by comparing the Saccharomyces cerevisiae and Candida albicans hsp90 gene sequences. Different sets of primers were designed to amplify and obtain overlapping DNA sequences of the C. tropicalis gene. PCR was carried out on genomic DNA of Candida tropicalis and the PCR products were cloned into suitable vector molecules for sequencing. In this way, a 2070-basepair sequence of the C. tropicalis hsp90 gene was obtained. The PCR-based approach proved to be an easier method of obtain the sequence of a highly conserved gene, as compared to more conventional methods. (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)35-38
Number of pages4
JournalFEMS Immunology and Medical Microbiology
Volume29
Issue number1
DOIs
Publication statusPublished - Sep 2000
Externally publishedYes

Fingerprint

Candida tropicalis
HSP90 Heat-Shock Proteins
Polymerase Chain Reaction
Genes
Candida albicans
Saccharomyces cerevisiae
DNA

Keywords

  • Candida tropicalis
  • Heat shock protein 90
  • Polymerase chain reaction
  • Sequencing

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

A PCR-based approach to sequence the Candida tropicalis HSP90 gene. / Santhanam, Jacinta; Burnie, James P.

In: FEMS Immunology and Medical Microbiology, Vol. 29, No. 1, 09.2000, p. 35-38.

Research output: Contribution to journalArticle

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