A novel high throughput method based on the DPPH dry reagent array for determination of antioxidant activity

Khalid Hamid Musa, Aminah Abdullah, Bambang Kuswandi, M. Amrun Hidayat

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A stable chromogenic radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) is commonly used for the determination of antioxidant activity. In this paper, DPPH was dried into 96 well microplate to produce DPPH dry reagent array plate, based on which the highly sensitive and high throughput determination of antioxidant activities was achieved. The spectrophotometric characterization of the microplate containing dried or fresh DPPH free radicals was reported. The response of the DPPH dry reagent array towards different standard antioxidants was studied. The reaction for DPPH in fresh or dry reagent array with Trolox was reported and compared. The DPPH dry reagent array was used to study the antioxidant activity of banana, green tea, pink guava, and honeydew and the results were compared to the samples reacted with freshly prepared DPPH. The proposed method is comparable to the classical DPPH method, more convenient, simple to operate with minimal solvent required and excellent sensitivity.

Original languageEnglish
Pages (from-to)4102-4106
Number of pages5
JournalFood Chemistry
Volume141
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

Antioxidants
antioxidant activity
Throughput
methodology
Psidium
Chromogenics
2,2-diphenyl-1-picrylhydrazyl
Musa
guavas
honeydew
green tea
Tea
bananas
Free Radicals
antioxidants

Keywords

  • 96 well microplate
  • Antioxidant activity
  • DPPH free radicals
  • Dry reagent array

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

A novel high throughput method based on the DPPH dry reagent array for determination of antioxidant activity. / Musa, Khalid Hamid; Abdullah, Aminah; Kuswandi, Bambang; Hidayat, M. Amrun.

In: Food Chemistry, Vol. 141, No. 4, 2013, p. 4102-4106.

Research output: Contribution to journalArticle

Musa, Khalid Hamid ; Abdullah, Aminah ; Kuswandi, Bambang ; Hidayat, M. Amrun. / A novel high throughput method based on the DPPH dry reagent array for determination of antioxidant activity. In: Food Chemistry. 2013 ; Vol. 141, No. 4. pp. 4102-4106.
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