A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Clostridium butyricum isolated from a microbial fuel cell

Hyung Soo Park, Byung Hong Kim, Hyo Suk Kim, Hyung Joo Kim, Gwang Tae Kim, Mia Kim, In Seop Chang, Yong Keun Park, Hyo Ihl Chang

Research output: Contribution to journalArticle

371 Citations (Scopus)

Abstract

An obligatory anaerobic bacterium was isolated from a mediator-less microbial fuel cell using starch processing wastewater as the fuel and designated as EG3. The isolate was Gram-positive, motile and rod (2.8-3.0 μm long, 0.5-0.6 μm wide). The partial 16S rRNA gene sequence and analysis of the cellular fatty acids profile suggested that EG3 clusters with Clostridium sub-phylum and exhibited the highest similarity (98%) with Clostridium butyricum. The temperature and pH optimum for growth were 37°C and 7.0, respectively. The major products of glucose and glucose/Fe(O)OH metabolism were lactate, formate, butyrate, acetate, CO2 and H2. Growth was faster at the initial phase and the cell yield was higher when the medium was supplemented with Fe(O)OH than without Fe(O)OH. These results suggest that Fe(III) ion is utilised as an electron sink. Cyclic voltammetry showed that Clostridium butyricum EG3 cells were electrochemically active. It is a novel characteristic of strict anaerobic Gram-positive bacteria.

Original languageEnglish
Pages (from-to)297-306
Number of pages10
JournalAnaerobe
Volume7
Issue number6
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Clostridium butyricum
Bioelectric Energy Sources
formic acid
Anaerobic Bacteria
Gram-Positive Rods
Bacteria
Glucose
Clostridium
Butyrates
Gram-Positive Bacteria
Growth
Waste Water
rRNA Genes
Starch
Sequence Analysis
Lactic Acid
Acetates
Fatty Acids
Electrons
Ions

Keywords

  • Clostridium sp. EG3
  • Cyclic voltammogram
  • Dissimilatory Fe(III) reduction
  • Electrochemically active bacteria
  • Microbial fuel cell

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Clostridium butyricum isolated from a microbial fuel cell. / Park, Hyung Soo; Kim, Byung Hong; Kim, Hyo Suk; Kim, Hyung Joo; Kim, Gwang Tae; Kim, Mia; Chang, In Seop; Park, Yong Keun; Chang, Hyo Ihl.

In: Anaerobe, Vol. 7, No. 6, 2001, p. 297-306.

Research output: Contribution to journalArticle

Park, Hyung Soo ; Kim, Byung Hong ; Kim, Hyo Suk ; Kim, Hyung Joo ; Kim, Gwang Tae ; Kim, Mia ; Chang, In Seop ; Park, Yong Keun ; Chang, Hyo Ihl. / A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Clostridium butyricum isolated from a microbial fuel cell. In: Anaerobe. 2001 ; Vol. 7, No. 6. pp. 297-306.
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