A novel electrochemical sensor for 17β-estradiol from molecularly imprinted polymeric microspheres and multi-walled carbon nanotubes grafted with gold nanoparticles

Dedi Futra, Yook Heng Lee, Mohamad Zudaidy Jaapar, Alizar Ulianas, Kasra Saeedfar, Ling Ling Tan @ Chong

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Molecularly imprinted polymers (MIPs) are generally a more stable material for sensing application. The high selectivity and sensitivity of MIPs for sensors can be achieved if the template molecule is imprinted in the polymer and this makes them an ideal alternative as a recognition element for sensors. A new electrochemical sensor based on molecularly imprinted polymeric microspheres (MIPs) and multi-walled carbon nanotube/gold nanoparticle (MIP-MWCNT-AuNP) modified carbon screen-printed electrodes (SPEs) for the rapid detection of 17β-estradiol (E) hormone in serum samples has been successfully developed. Hydrophobic MIPs were synthesized using photopolymerization in emulsion form. The multi-walled carbon nanotube grafted with gold nanoparticles was firstly deposited onto a carbon screen-printed electrode for the purpose of accelerating electron transfer to the surface of the electrode. The MIP microspheres specific to the 17β-estradiol hormone, prepared via a facile photopolymerization technique, were coated onto the MWCNT-AuNP modified SPE. The presence of 17β-estradiol in biological samples could be detected with the sensor via absorption of 17β-estradiol into the deposited MIPs and this was monitored by differential pulse voltammetry (DPV) at 0.6 V for the reduction of 17β-estradiol. Under optimal conditions, the sensor could detect the concentrations of 17β-estradiol from 1.0 × 10-15 to 1.0 × 10-6 M (R2 = 0.9921), with a detection limit of 2.5 × 10-16 M. The sensor based on MIP microspheres and the MWCNT-AuNP modified electrode demonstrated a stability of 55 days with good reproducibility (RSD <5%, n = 5) and regenerability (RSD <4%, n = 5). Using this sensor, the gender of the arowana fish determined via the level of 17β-estradiol using fish serum samples demonstrated good agreement with a conventional test kit based on the immuno-assay method.

Original languageEnglish
Pages (from-to)1381-1389
Number of pages9
JournalAnalytical Methods
Volume8
Issue number6
DOIs
Publication statusPublished - 14 Feb 2016

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Electrochemical sensors
Carbon Nanotubes
Microspheres
Gold
Estradiol
Carbon nanotubes
Polymers
Nanoparticles
Sensors
Electrodes
Photopolymerization
Hormones
Fish
Carbon
Voltammetry
Emulsions
Assays
Molecules
Electrons

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)

Cite this

A novel electrochemical sensor for 17β-estradiol from molecularly imprinted polymeric microspheres and multi-walled carbon nanotubes grafted with gold nanoparticles. / Futra, Dedi; Lee, Yook Heng; Jaapar, Mohamad Zudaidy; Ulianas, Alizar; Saeedfar, Kasra; Tan @ Chong, Ling Ling.

In: Analytical Methods, Vol. 8, No. 6, 14.02.2016, p. 1381-1389.

Research output: Contribution to journalArticle

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