Abstract
Maspine (Ananas comosus L.) is currently the most preferred pineapple variety in Malaysia due to its pleasant aroma and applicability in caning. Large quantities of plant materials are needed to fulfill the market demand which could not be obtained from the conventional breeding method. Hence, in vitro procedure was developed as an alternative method to improve the multiplication rate of this special variety. Sterilized explants were cultured on solidified Murashige and Skoog (MS) medium supplemented with various combinations of 6-benzylaminopurine (BAP) (1 to 5 mg/l) and α-naphthaleneacetic acid (NAA) (1 to 5 mg/l) hormones. Pineapple plant cultures required 5 mg/l BAP to significantly increase the shoot development during the in vitro stage. In addition, explants were subsequently sub-cultured on medium with 1 mg/l BAP which produced highest number of proliferated in vitro plantlets. The optimization of the conditions for shoot propagation was carried out in both liquid and solid medium by supplementing with 1 or 5 mg/l of BAP. MS liquid medium supplemented with 1 mg/l BAP produced the highest number of shoots (31) after 4 weeks. The number of shoots formed was increased to 204 after third sub-culture in liquid medium. Shoot proliferation was increased up to ninefold in liquid medium when compared to the cultures maintained on solid medium. This improved method of Maspine in vitro multiplication will serve as an alternative source of planting materials of this cultivar for subsistence and large-scale pineapple farmers.
| Original language | English |
|---|---|
| Pages (from-to) | 3859-3866 |
| Number of pages | 8 |
| Journal | African Journal of Biotechnology |
| Volume | 10 |
| Issue number | 19 |
| Publication status | Published - 9 May 2011 |
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Keywords
- α-naphthaleneacetic acid
- 6-benzylaminopurine
- In Vitro
- Liquid medium
- Pineapple
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Genetics
- Molecular Biology
- Agronomy and Crop Science
Cite this
A novel approach for rapid micropropagation of maspine pineapple (Ananas comosus L.) shoots using liquid shake culture system. / Zuraida, A. R.; Nurl Shahnadz, A. H.; Harteeni, A.; Roowi, S.; Che Mohd. Zain, Che Radziah; Sreeramanan, S.
In: African Journal of Biotechnology, Vol. 10, No. 19, 09.05.2011, p. 3859-3866.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel approach for rapid micropropagation of maspine pineapple (Ananas comosus L.) shoots using liquid shake culture system
AU - Zuraida, A. R.
AU - Nurl Shahnadz, A. H.
AU - Harteeni, A.
AU - Roowi, S.
AU - Che Mohd. Zain, Che Radziah
AU - Sreeramanan, S.
PY - 2011/5/9
Y1 - 2011/5/9
N2 - Maspine (Ananas comosus L.) is currently the most preferred pineapple variety in Malaysia due to its pleasant aroma and applicability in caning. Large quantities of plant materials are needed to fulfill the market demand which could not be obtained from the conventional breeding method. Hence, in vitro procedure was developed as an alternative method to improve the multiplication rate of this special variety. Sterilized explants were cultured on solidified Murashige and Skoog (MS) medium supplemented with various combinations of 6-benzylaminopurine (BAP) (1 to 5 mg/l) and α-naphthaleneacetic acid (NAA) (1 to 5 mg/l) hormones. Pineapple plant cultures required 5 mg/l BAP to significantly increase the shoot development during the in vitro stage. In addition, explants were subsequently sub-cultured on medium with 1 mg/l BAP which produced highest number of proliferated in vitro plantlets. The optimization of the conditions for shoot propagation was carried out in both liquid and solid medium by supplementing with 1 or 5 mg/l of BAP. MS liquid medium supplemented with 1 mg/l BAP produced the highest number of shoots (31) after 4 weeks. The number of shoots formed was increased to 204 after third sub-culture in liquid medium. Shoot proliferation was increased up to ninefold in liquid medium when compared to the cultures maintained on solid medium. This improved method of Maspine in vitro multiplication will serve as an alternative source of planting materials of this cultivar for subsistence and large-scale pineapple farmers.
AB - Maspine (Ananas comosus L.) is currently the most preferred pineapple variety in Malaysia due to its pleasant aroma and applicability in caning. Large quantities of plant materials are needed to fulfill the market demand which could not be obtained from the conventional breeding method. Hence, in vitro procedure was developed as an alternative method to improve the multiplication rate of this special variety. Sterilized explants were cultured on solidified Murashige and Skoog (MS) medium supplemented with various combinations of 6-benzylaminopurine (BAP) (1 to 5 mg/l) and α-naphthaleneacetic acid (NAA) (1 to 5 mg/l) hormones. Pineapple plant cultures required 5 mg/l BAP to significantly increase the shoot development during the in vitro stage. In addition, explants were subsequently sub-cultured on medium with 1 mg/l BAP which produced highest number of proliferated in vitro plantlets. The optimization of the conditions for shoot propagation was carried out in both liquid and solid medium by supplementing with 1 or 5 mg/l of BAP. MS liquid medium supplemented with 1 mg/l BAP produced the highest number of shoots (31) after 4 weeks. The number of shoots formed was increased to 204 after third sub-culture in liquid medium. Shoot proliferation was increased up to ninefold in liquid medium when compared to the cultures maintained on solid medium. This improved method of Maspine in vitro multiplication will serve as an alternative source of planting materials of this cultivar for subsistence and large-scale pineapple farmers.
KW - α-naphthaleneacetic acid
KW - 6-benzylaminopurine
KW - In Vitro
KW - Liquid medium
KW - Pineapple
UR - http://www.scopus.com/inward/record.url?scp=79957584866&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79957584866&partnerID=8YFLogxK
M3 - Article
VL - 10
SP - 3859
EP - 3866
JO - African Journal of Biotechnology
JF - African Journal of Biotechnology
SN - 1684-5315
IS - 19
ER -