Kaedah spektrofotometrik ultra violet-cahaya nampak baharu untuk penentuan akrilamida secara kuantitatif melalui proses hidrolisis

Translated title of the contribution: A new ultra violet-visible spectrophotometric method for quantitative determination of acrylamide via hydrolysis process

Yee May Chong, Musa Ahmad, Yook Heng Lee

Research output: Contribution to journalArticle

Abstract

This paper reported the results for quantitative determination of acrylamide via hydrolysis process using ultra violet-visible (UV-Vis) spectrophotometric method. This quantitative determination started with hydrolysing acrylamide in a strong basic condition to yield ammonia and acid salt. The optimum conditions of hydrolysis (concentration of the base used and time for hydrolysis) were also determined. From this study, the optimum conditions to hydrolyse acrylamide were achieved using 6.0 M of sodium hydroxide (NaOH) for 10 minutes. The hydrolysis process was characterised by monitoring the ammonia produced using Nessler’s reagent through the formation of yellow colouration in the presence of ammonia. After the optimisation of hydrolysis process, the characterisation of all parameters including concentration of Nessler’s reagent used, reproducibility, dynamic range, and interference ions were studied. Linear dynamic ranges from 0-10 ppm acrylamide with limit of detection (LOD) of 0.074 ppm were obtained when 3.0 mM Nessler’s reagent was used. The relative standard deviations (RSD) for reproducibility were 2.8-3.3%. No significant interference from cations such as Na + , K + , Ca 2+ during quantitative analysis of acrylamide, but ions such as Fe 3+ and NH 3 affected the analysis.

Original languageMalay
Pages (from-to)14-22
Number of pages9
JournalMalaysian Journal of Analytical Sciences
Volume23
Issue number1
DOIs
Publication statusPublished - 1 Feb 2019

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Acrylamide
Hydrolysis
Ammonia
Ions
Sodium Hydroxide
Cations
Salts
Acids
Monitoring
Chemical analysis

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Kaedah spektrofotometrik ultra violet-cahaya nampak baharu untuk penentuan akrilamida secara kuantitatif melalui proses hidrolisis",
abstract = "This paper reported the results for quantitative determination of acrylamide via hydrolysis process using ultra violet-visible (UV-Vis) spectrophotometric method. This quantitative determination started with hydrolysing acrylamide in a strong basic condition to yield ammonia and acid salt. The optimum conditions of hydrolysis (concentration of the base used and time for hydrolysis) were also determined. From this study, the optimum conditions to hydrolyse acrylamide were achieved using 6.0 M of sodium hydroxide (NaOH) for 10 minutes. The hydrolysis process was characterised by monitoring the ammonia produced using Nessler’s reagent through the formation of yellow colouration in the presence of ammonia. After the optimisation of hydrolysis process, the characterisation of all parameters including concentration of Nessler’s reagent used, reproducibility, dynamic range, and interference ions were studied. Linear dynamic ranges from 0-10 ppm acrylamide with limit of detection (LOD) of 0.074 ppm were obtained when 3.0 mM Nessler’s reagent was used. The relative standard deviations (RSD) for reproducibility were 2.8-3.3{\%}. No significant interference from cations such as Na + , K + , Ca 2+ during quantitative analysis of acrylamide, but ions such as Fe 3+ and NH 3 affected the analysis.",
keywords = "Acrylamide, Hydrolysis, Nessler’s reagent",
author = "Chong, {Yee May} and Musa Ahmad and Lee, {Yook Heng}",
year = "2019",
month = "2",
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language = "Malay",
volume = "23",
pages = "14--22",
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AU - Ahmad, Musa

AU - Lee, Yook Heng

PY - 2019/2/1

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N2 - This paper reported the results for quantitative determination of acrylamide via hydrolysis process using ultra violet-visible (UV-Vis) spectrophotometric method. This quantitative determination started with hydrolysing acrylamide in a strong basic condition to yield ammonia and acid salt. The optimum conditions of hydrolysis (concentration of the base used and time for hydrolysis) were also determined. From this study, the optimum conditions to hydrolyse acrylamide were achieved using 6.0 M of sodium hydroxide (NaOH) for 10 minutes. The hydrolysis process was characterised by monitoring the ammonia produced using Nessler’s reagent through the formation of yellow colouration in the presence of ammonia. After the optimisation of hydrolysis process, the characterisation of all parameters including concentration of Nessler’s reagent used, reproducibility, dynamic range, and interference ions were studied. Linear dynamic ranges from 0-10 ppm acrylamide with limit of detection (LOD) of 0.074 ppm were obtained when 3.0 mM Nessler’s reagent was used. The relative standard deviations (RSD) for reproducibility were 2.8-3.3%. No significant interference from cations such as Na + , K + , Ca 2+ during quantitative analysis of acrylamide, but ions such as Fe 3+ and NH 3 affected the analysis.

AB - This paper reported the results for quantitative determination of acrylamide via hydrolysis process using ultra violet-visible (UV-Vis) spectrophotometric method. This quantitative determination started with hydrolysing acrylamide in a strong basic condition to yield ammonia and acid salt. The optimum conditions of hydrolysis (concentration of the base used and time for hydrolysis) were also determined. From this study, the optimum conditions to hydrolyse acrylamide were achieved using 6.0 M of sodium hydroxide (NaOH) for 10 minutes. The hydrolysis process was characterised by monitoring the ammonia produced using Nessler’s reagent through the formation of yellow colouration in the presence of ammonia. After the optimisation of hydrolysis process, the characterisation of all parameters including concentration of Nessler’s reagent used, reproducibility, dynamic range, and interference ions were studied. Linear dynamic ranges from 0-10 ppm acrylamide with limit of detection (LOD) of 0.074 ppm were obtained when 3.0 mM Nessler’s reagent was used. The relative standard deviations (RSD) for reproducibility were 2.8-3.3%. No significant interference from cations such as Na + , K + , Ca 2+ during quantitative analysis of acrylamide, but ions such as Fe 3+ and NH 3 affected the analysis.

KW - Acrylamide

KW - Hydrolysis

KW - Nessler’s reagent

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