A new strain of docosahexaenoic acid producing microalga from Malaysian coastal waters

Vidyah Manikan, Mohamed Yusuf Mohamed Nazir, Mohd. Sahaid Kalil, Mohd Hafez Mohd Isa, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff, Aidil Abdul Hamid

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Thraustochytrids are marine protists belonging to the class Labyrinthula of the kingdom Chromista. This group of microbes is considered a promising alternative source of high-valued omega-3 oils, especially docosahexaenoic acid (DHA, C22:6. n3), replacing fish oil which is the current major source of DHA and eicosapentaenoic acid (EPA, C20:5. n3). Our new isolate, SW1, was considered to be an Aurantiochytrium based on its morphology, fatty acid profile and molecular phylogenetic analysis. Microscopic observations revealed that SW1 has high similarity to Aurantiochytrium limacinum ATCC MYA-1381 (SR21), possessing spherical vegetative cells which undergo repeated bipartition to form diads, tetrads, octads and zoosporangia that release motile zoospores. When cultivated in four different media, SW1 produced the highest biomass (13.17. g/L) and DHA (3.6. g/L) in a medium composed of glucose, sea salt, monosodium glutamate (MSG) and yeast extract. Visualization of lipid droplet development in SW1 using Sudan Black B dye revealed that lipid droplets enlarge to occupy almost the entire cell volume within 72 to 96. h of cultivation. This strain was also found to be able to utilize various saccharides as carbon source. The results of this study show that Aurantiochytrium sp. SW1 is a potential candidate to be developed as commercial microbial DHA producer.

Original languageEnglish
Pages (from-to)40-47
Number of pages8
JournalAlgal Research
Volume9
DOIs
Publication statusPublished - 1 May 2015

Fingerprint

docosahexaenoic acid
droplets
coastal water
monosodium glutamate
vegetative cells
zoospores
Sudan
yeast extract
lipids
eicosapentaenoic acid
fish oils
dyes
fatty acid composition
carbohydrates
salts
microorganisms
oils
glucose
carbon
biomass

Keywords

  • Aurantiochytrium
  • Docosahexaenoic acid
  • Omega-3 alternative
  • Polyunsaturated fatty acids
  • Thraustochytrid

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

A new strain of docosahexaenoic acid producing microalga from Malaysian coastal waters. / Manikan, Vidyah; Nazir, Mohamed Yusuf Mohamed; Kalil, Mohd. Sahaid; Isa, Mohd Hafez Mohd; Kader, Abdul Jalil Abdul; Wan Yusoff, Wan Mohtar; Abdul Hamid, Aidil.

In: Algal Research, Vol. 9, 01.05.2015, p. 40-47.

Research output: Contribution to journalArticle

Manikan, Vidyah ; Nazir, Mohamed Yusuf Mohamed ; Kalil, Mohd. Sahaid ; Isa, Mohd Hafez Mohd ; Kader, Abdul Jalil Abdul ; Wan Yusoff, Wan Mohtar ; Abdul Hamid, Aidil. / A new strain of docosahexaenoic acid producing microalga from Malaysian coastal waters. In: Algal Research. 2015 ; Vol. 9. pp. 40-47.
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