A new method for the isolation and purification of Lawsone from Lawsonia Inermis and its ROS inhibitory activity

Syed Muhammad Ghufran Saeed, Syed Asad Sayeed, Seema Ashraf, Shahina Naz, Rahmanullah Siddiqi, Rashida Ali, Osman Mesaik Mohammed Ahmed Hassan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Lawsone a coloring component of the Henna (Lawsonia inermis) leaves was found to bind with proteins. Present study has suggested a new rapid method for the isolation of lawsone from L. inermis through the calcium ion using the Flash and Disc Counter Current Chromatography (DCC). The purified pigment structurally characterized by 2D, TLC and NMR spectroscopy, was found to be active against reactive oxygen species (ROS) by Chemeluminiscence assay using lymphocytes. Purified lawsone showed remarkable inhibitory activity on the oxidative burst response of the whole blood, polymorphonuclear cells (PMNCs), Mononuclear cells (MNCs) with IC50 as 13 ±1.6 μg/ml, 11±2.7 μg/ml. and 10±4.2 μg/ml respectively.

Original languageEnglish
Pages (from-to)1431-1436
Number of pages6
JournalPakistan Journal of Botany
Volume45
Issue number4
Publication statusPublished - Aug 2013
Externally publishedYes

Fingerprint

Lawsonia inermis
reactive oxygen species
countercurrent chromatography
rapid methods
inhibitory concentration 50
nuclear magnetic resonance spectroscopy
lymphocytes
pigments
cells
ions
calcium
blood
assays
methodology
leaves
proteins

ASJC Scopus subject areas

  • Plant Science

Cite this

A new method for the isolation and purification of Lawsone from Lawsonia Inermis and its ROS inhibitory activity. / Saeed, Syed Muhammad Ghufran; Sayeed, Syed Asad; Ashraf, Seema; Naz, Shahina; Siddiqi, Rahmanullah; Ali, Rashida; Mohammed Ahmed Hassan, Osman Mesaik.

In: Pakistan Journal of Botany, Vol. 45, No. 4, 08.2013, p. 1431-1436.

Research output: Contribution to journalArticle

Saeed SMG, Sayeed SA, Ashraf S, Naz S, Siddiqi R, Ali R et al. A new method for the isolation and purification of Lawsone from Lawsonia Inermis and its ROS inhibitory activity. Pakistan Journal of Botany. 2013 Aug;45(4):1431-1436.
Saeed, Syed Muhammad Ghufran ; Sayeed, Syed Asad ; Ashraf, Seema ; Naz, Shahina ; Siddiqi, Rahmanullah ; Ali, Rashida ; Mohammed Ahmed Hassan, Osman Mesaik. / A new method for the isolation and purification of Lawsone from Lawsonia Inermis and its ROS inhibitory activity. In: Pakistan Journal of Botany. 2013 ; Vol. 45, No. 4. pp. 1431-1436.
@article{bd7c7b9a32bd4ec495ca246e1811fd4f,
title = "A new method for the isolation and purification of Lawsone from Lawsonia Inermis and its ROS inhibitory activity",
abstract = "Lawsone a coloring component of the Henna (Lawsonia inermis) leaves was found to bind with proteins. Present study has suggested a new rapid method for the isolation of lawsone from L. inermis through the calcium ion using the Flash and Disc Counter Current Chromatography (DCC). The purified pigment structurally characterized by 2D, TLC and NMR spectroscopy, was found to be active against reactive oxygen species (ROS) by Chemeluminiscence assay using lymphocytes. Purified lawsone showed remarkable inhibitory activity on the oxidative burst response of the whole blood, polymorphonuclear cells (PMNCs), Mononuclear cells (MNCs) with IC50 as 13 ±1.6 μg/ml, 11±2.7 μg/ml. and 10±4.2 μg/ml respectively.",
author = "Saeed, {Syed Muhammad Ghufran} and Sayeed, {Syed Asad} and Seema Ashraf and Shahina Naz and Rahmanullah Siddiqi and Rashida Ali and {Mohammed Ahmed Hassan}, {Osman Mesaik}",
year = "2013",
month = "8",
language = "English",
volume = "45",
pages = "1431--1436",
journal = "Pakistan Journal of Botany",
issn = "0556-3321",
publisher = "Pakistan Botanical Society",
number = "4",

}

TY - JOUR

T1 - A new method for the isolation and purification of Lawsone from Lawsonia Inermis and its ROS inhibitory activity

AU - Saeed, Syed Muhammad Ghufran

AU - Sayeed, Syed Asad

AU - Ashraf, Seema

AU - Naz, Shahina

AU - Siddiqi, Rahmanullah

AU - Ali, Rashida

AU - Mohammed Ahmed Hassan, Osman Mesaik

PY - 2013/8

Y1 - 2013/8

N2 - Lawsone a coloring component of the Henna (Lawsonia inermis) leaves was found to bind with proteins. Present study has suggested a new rapid method for the isolation of lawsone from L. inermis through the calcium ion using the Flash and Disc Counter Current Chromatography (DCC). The purified pigment structurally characterized by 2D, TLC and NMR spectroscopy, was found to be active against reactive oxygen species (ROS) by Chemeluminiscence assay using lymphocytes. Purified lawsone showed remarkable inhibitory activity on the oxidative burst response of the whole blood, polymorphonuclear cells (PMNCs), Mononuclear cells (MNCs) with IC50 as 13 ±1.6 μg/ml, 11±2.7 μg/ml. and 10±4.2 μg/ml respectively.

AB - Lawsone a coloring component of the Henna (Lawsonia inermis) leaves was found to bind with proteins. Present study has suggested a new rapid method for the isolation of lawsone from L. inermis through the calcium ion using the Flash and Disc Counter Current Chromatography (DCC). The purified pigment structurally characterized by 2D, TLC and NMR spectroscopy, was found to be active against reactive oxygen species (ROS) by Chemeluminiscence assay using lymphocytes. Purified lawsone showed remarkable inhibitory activity on the oxidative burst response of the whole blood, polymorphonuclear cells (PMNCs), Mononuclear cells (MNCs) with IC50 as 13 ±1.6 μg/ml, 11±2.7 μg/ml. and 10±4.2 μg/ml respectively.

UR - http://www.scopus.com/inward/record.url?scp=84879970955&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879970955&partnerID=8YFLogxK

M3 - Article

VL - 45

SP - 1431

EP - 1436

JO - Pakistan Journal of Botany

JF - Pakistan Journal of Botany

SN - 0556-3321

IS - 4

ER -