A new Laccase based biosensor for tartrazine

Siti Zulaikha Mazlan, Yook Heng Lee, Sharina Abu Hanifah

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Laccase enzyme, a commonly used enzyme for the construction of biosensors for phenolic compounds was used for the first time to develop a new biosensor for the determination of the azo-dye tartrazine. The electrochemical biosensor was based on the immobilization of laccase on functionalized methacrylate-acrylate microspheres. The biosensor membrane is a composite of the laccase conjugated microspheres and gold nanoparticles (AuNPs) coated on a carbon-paste screen-printed electrode. The reaction involving tartrazine can be catalyzed by laccase enzyme, where the current change was measured by differential pulse voltammetry (DPV) at 1.1 V. The anodic peak current was linear within the tartrazine concentration range of 0.2 to 14 μM (R2= 0.979) and the detection limit was 0.04 μM. Common food ingredients or additives such as glucose, sucrose, ascorbic acid, phenol and sunset yellow did not interfere with the biosensor response. Furthermore, the biosensor response was stable up to 30 days of storage period at 4 °C. Foods and beverage were used as real samples for the biosensor validation. The biosensor response to tartrazine showed no significant difference with a standard HPLC method for tartrazine analysis.

Original languageEnglish
Article number2859
JournalSensors (Switzerland)
Volume17
Issue number12
DOIs
Publication statusPublished - 9 Dec 2017

Fingerprint

Tartrazine
Laccase
Biosensing Techniques
bioinstrumentation
Biosensors
enzymes
Enzymes
Microspheres
food
Azo Compounds
beverages
Food and Beverages
sunset
Azo dyes
Beverages
Methacrylates
ascorbic acid
sucrose
Ascorbic acid
Sugar (sucrose)

Keywords

  • AuNPs
  • Electrochemical sensor
  • Enzymatic biosensor
  • Methacrylate-acrylate microspheres
  • Tartrazine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Biochemistry
  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

A new Laccase based biosensor for tartrazine. / Mazlan, Siti Zulaikha; Lee, Yook Heng; Abu Hanifah, Sharina.

In: Sensors (Switzerland), Vol. 17, No. 12, 2859, 09.12.2017.

Research output: Contribution to journalArticle

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