A mouse igM monoclonal antibody recognizes breast and colon cancer

Ainul Fajariah A Bakar, Noorjahan Banu Alitheen, Yeap Swee Keong, Muhajir Hamid, Siti Aishah M Ali, Abdul Manaf Ali

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Hybridoma clone C3A8, which is a fusion product between splenic lymphocytes of Balb/c mice immunized with MCF7 breast carcinoma cells and SP2/0 myelomas, was produced and characterized. A stable clone that secreted IgM monoclonal antibody (MAb) with k light chain was obtained through limiting dilutions. Cell-ELISA screening, flow cytometry analysis, and immunofluorescence staining revealed that the MAb C3A8 had bound specifically and strongly to MCF7 and HT29 but cross reacted weakly or not on HeLa cell line. The MAb C3A8 reacted positively with paraffin-embedded tissues of human breast and colon cancers but there were no positive reactions on normal tissues. Western blot analysis showed the MAb recognized a 55kDa protein, which was present in the extract of MCF7 and HT29 cell lines. Our results demonstrated that MAb C3A8 could be used for basic and clinical research of breast and colon cancers.

    Original languageEnglish
    Pages (from-to)199-203
    Number of pages5
    JournalHybridoma
    Volume28
    Issue number3
    DOIs
    Publication statusPublished - 1 Jun 2009

    Fingerprint

    Colonic Neoplasms
    Monoclonal Antibodies
    Breast Neoplasms
    Clone Cells
    Cell Line
    HT29 Cells
    MCF-7 Cells
    Hybridomas
    HeLa Cells
    Paraffin
    Fluorescent Antibody Technique
    Immunoglobulin M
    Flow Cytometry
    Western Blotting
    Enzyme-Linked Immunosorbent Assay
    Lymphocytes
    Staining and Labeling
    Light
    Research
    Proteins

    ASJC Scopus subject areas

    • Immunology
    • Immunology and Allergy

    Cite this

    Bakar, A. F. A., Alitheen, N. B., Keong, Y. S., Hamid, M., Ali, S. A. M., & Ali, A. M. (2009). A mouse igM monoclonal antibody recognizes breast and colon cancer. Hybridoma, 28(3), 199-203. https://doi.org/10.1089/hyb.2007.0531

    A mouse igM monoclonal antibody recognizes breast and colon cancer. / Bakar, Ainul Fajariah A; Alitheen, Noorjahan Banu; Keong, Yeap Swee; Hamid, Muhajir; Ali, Siti Aishah M; Ali, Abdul Manaf.

    In: Hybridoma, Vol. 28, No. 3, 01.06.2009, p. 199-203.

    Research output: Contribution to journalArticle

    Bakar, AFA, Alitheen, NB, Keong, YS, Hamid, M, Ali, SAM & Ali, AM 2009, 'A mouse igM monoclonal antibody recognizes breast and colon cancer', Hybridoma, vol. 28, no. 3, pp. 199-203. https://doi.org/10.1089/hyb.2007.0531
    Bakar AFA, Alitheen NB, Keong YS, Hamid M, Ali SAM, Ali AM. A mouse igM monoclonal antibody recognizes breast and colon cancer. Hybridoma. 2009 Jun 1;28(3):199-203. https://doi.org/10.1089/hyb.2007.0531
    Bakar, Ainul Fajariah A ; Alitheen, Noorjahan Banu ; Keong, Yeap Swee ; Hamid, Muhajir ; Ali, Siti Aishah M ; Ali, Abdul Manaf. / A mouse igM monoclonal antibody recognizes breast and colon cancer. In: Hybridoma. 2009 ; Vol. 28, No. 3. pp. 199-203.
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