A molybdenum-reducing Bacillus sp. strain zeid 14 in Soils from Sudan that could grow on amides and acetonitrile

A. S. Mohd Adnan, I. M. Abu Zeid, S. A. Ahmad, M. I.E. Halmi, Siti Rozaimah Sheikh Abdullah, N. A. Masdor, M. S. Shukor, M. Y. Shukor

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Agricultural and industrial activities contribute most to pollutants found globally, and bioremediation of these pollutants is being intensely sought. We have isolated a molybdenum-reducing bacterium from agricultural soil for bioremediation purposes. The bacterium was grown in low phosphate medium supplemented with molybdate in a microplate format. The molybdenum-reducing bacterium was then further screened for amide-degrading properties. The bacterium was able to use acrylamide as a source of electron donor for reduction, and was able to grow on acrylamide, acetamide and acetonitrile. The growth parameters obtained according to the modified Gompertz model were lag periods of 0.468, 0.979 and 1.53 d and maximum specific growth rates of 1.165, 0.932, 0.842 d-1 for acrylamide, acetamide and acetonitrile respectively. Optimal conditions for molybdate reduction included glucose, pH between 6.0 and 6.8, temperature between 25° C and 34° C, and phosphate and molybdate concentrations between 5 and 7.5 mM and 10 and 20 mM, respectively. The Mo-blue exhibited a unique absorption spectrum closely resembling a reduced phosphomolybdate. Mo-blue production was inhibited by the heavy metals copper, mercury, silver, chromium and cadmium. The bacterium was identified as Bacillus sp. strain Zeid 14. The bacterium will be very useful for bioremediation of sites contaminated with molybdenum and amides.

Original languageEnglish
Pages (from-to)111-134
Number of pages24
JournalMalaysian Journal of Soil Science
Volume20
Publication statusPublished - 1 Dec 2016

Fingerprint

molybdenum
Bacillus (bacteria)
Sudan
amides
molybdates
bacterium
acrylamides
bacteria
bioremediation
acetamides
soil
pollutants
phosphate
phosphates
pollutant
microplate
absorption spectrum
agricultural soils
chromium
agricultural soil

Keywords

  • Acetonitrile
  • Acrylamide
  • Bacillus sp.
  • Molybdenum blue
  • Molybdenum-reducing bacterium

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science

Cite this

Mohd Adnan, A. S., Abu Zeid, I. M., Ahmad, S. A., Halmi, M. I. E., Sheikh Abdullah, S. R., Masdor, N. A., ... Shukor, M. Y. (2016). A molybdenum-reducing Bacillus sp. strain zeid 14 in Soils from Sudan that could grow on amides and acetonitrile. Malaysian Journal of Soil Science, 20, 111-134.

A molybdenum-reducing Bacillus sp. strain zeid 14 in Soils from Sudan that could grow on amides and acetonitrile. / Mohd Adnan, A. S.; Abu Zeid, I. M.; Ahmad, S. A.; Halmi, M. I.E.; Sheikh Abdullah, Siti Rozaimah; Masdor, N. A.; Shukor, M. S.; Shukor, M. Y.

In: Malaysian Journal of Soil Science, Vol. 20, 01.12.2016, p. 111-134.

Research output: Contribution to journalArticle

Mohd Adnan, AS, Abu Zeid, IM, Ahmad, SA, Halmi, MIE, Sheikh Abdullah, SR, Masdor, NA, Shukor, MS & Shukor, MY 2016, 'A molybdenum-reducing Bacillus sp. strain zeid 14 in Soils from Sudan that could grow on amides and acetonitrile', Malaysian Journal of Soil Science, vol. 20, pp. 111-134.
Mohd Adnan, A. S. ; Abu Zeid, I. M. ; Ahmad, S. A. ; Halmi, M. I.E. ; Sheikh Abdullah, Siti Rozaimah ; Masdor, N. A. ; Shukor, M. S. ; Shukor, M. Y. / A molybdenum-reducing Bacillus sp. strain zeid 14 in Soils from Sudan that could grow on amides and acetonitrile. In: Malaysian Journal of Soil Science. 2016 ; Vol. 20. pp. 111-134.
@article{461a9e1330974b138d49e43766b8ab42,
title = "A molybdenum-reducing Bacillus sp. strain zeid 14 in Soils from Sudan that could grow on amides and acetonitrile",
abstract = "Agricultural and industrial activities contribute most to pollutants found globally, and bioremediation of these pollutants is being intensely sought. We have isolated a molybdenum-reducing bacterium from agricultural soil for bioremediation purposes. The bacterium was grown in low phosphate medium supplemented with molybdate in a microplate format. The molybdenum-reducing bacterium was then further screened for amide-degrading properties. The bacterium was able to use acrylamide as a source of electron donor for reduction, and was able to grow on acrylamide, acetamide and acetonitrile. The growth parameters obtained according to the modified Gompertz model were lag periods of 0.468, 0.979 and 1.53 d and maximum specific growth rates of 1.165, 0.932, 0.842 d-1 for acrylamide, acetamide and acetonitrile respectively. Optimal conditions for molybdate reduction included glucose, pH between 6.0 and 6.8, temperature between 25° C and 34° C, and phosphate and molybdate concentrations between 5 and 7.5 mM and 10 and 20 mM, respectively. The Mo-blue exhibited a unique absorption spectrum closely resembling a reduced phosphomolybdate. Mo-blue production was inhibited by the heavy metals copper, mercury, silver, chromium and cadmium. The bacterium was identified as Bacillus sp. strain Zeid 14. The bacterium will be very useful for bioremediation of sites contaminated with molybdenum and amides.",
keywords = "Acetonitrile, Acrylamide, Bacillus sp., Molybdenum blue, Molybdenum-reducing bacterium",
author = "{Mohd Adnan}, {A. S.} and {Abu Zeid}, {I. M.} and Ahmad, {S. A.} and Halmi, {M. I.E.} and {Sheikh Abdullah}, {Siti Rozaimah} and Masdor, {N. A.} and Shukor, {M. S.} and Shukor, {M. Y.}",
year = "2016",
month = "12",
day = "1",
language = "English",
volume = "20",
pages = "111--134",
journal = "Malaysian Journal of Soil Science",
issn = "1394-7990",
publisher = "Malaysian Society of Soil Science",

}

TY - JOUR

T1 - A molybdenum-reducing Bacillus sp. strain zeid 14 in Soils from Sudan that could grow on amides and acetonitrile

AU - Mohd Adnan, A. S.

AU - Abu Zeid, I. M.

AU - Ahmad, S. A.

AU - Halmi, M. I.E.

AU - Sheikh Abdullah, Siti Rozaimah

AU - Masdor, N. A.

AU - Shukor, M. S.

AU - Shukor, M. Y.

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Agricultural and industrial activities contribute most to pollutants found globally, and bioremediation of these pollutants is being intensely sought. We have isolated a molybdenum-reducing bacterium from agricultural soil for bioremediation purposes. The bacterium was grown in low phosphate medium supplemented with molybdate in a microplate format. The molybdenum-reducing bacterium was then further screened for amide-degrading properties. The bacterium was able to use acrylamide as a source of electron donor for reduction, and was able to grow on acrylamide, acetamide and acetonitrile. The growth parameters obtained according to the modified Gompertz model were lag periods of 0.468, 0.979 and 1.53 d and maximum specific growth rates of 1.165, 0.932, 0.842 d-1 for acrylamide, acetamide and acetonitrile respectively. Optimal conditions for molybdate reduction included glucose, pH between 6.0 and 6.8, temperature between 25° C and 34° C, and phosphate and molybdate concentrations between 5 and 7.5 mM and 10 and 20 mM, respectively. The Mo-blue exhibited a unique absorption spectrum closely resembling a reduced phosphomolybdate. Mo-blue production was inhibited by the heavy metals copper, mercury, silver, chromium and cadmium. The bacterium was identified as Bacillus sp. strain Zeid 14. The bacterium will be very useful for bioremediation of sites contaminated with molybdenum and amides.

AB - Agricultural and industrial activities contribute most to pollutants found globally, and bioremediation of these pollutants is being intensely sought. We have isolated a molybdenum-reducing bacterium from agricultural soil for bioremediation purposes. The bacterium was grown in low phosphate medium supplemented with molybdate in a microplate format. The molybdenum-reducing bacterium was then further screened for amide-degrading properties. The bacterium was able to use acrylamide as a source of electron donor for reduction, and was able to grow on acrylamide, acetamide and acetonitrile. The growth parameters obtained according to the modified Gompertz model were lag periods of 0.468, 0.979 and 1.53 d and maximum specific growth rates of 1.165, 0.932, 0.842 d-1 for acrylamide, acetamide and acetonitrile respectively. Optimal conditions for molybdate reduction included glucose, pH between 6.0 and 6.8, temperature between 25° C and 34° C, and phosphate and molybdate concentrations between 5 and 7.5 mM and 10 and 20 mM, respectively. The Mo-blue exhibited a unique absorption spectrum closely resembling a reduced phosphomolybdate. Mo-blue production was inhibited by the heavy metals copper, mercury, silver, chromium and cadmium. The bacterium was identified as Bacillus sp. strain Zeid 14. The bacterium will be very useful for bioremediation of sites contaminated with molybdenum and amides.

KW - Acetonitrile

KW - Acrylamide

KW - Bacillus sp.

KW - Molybdenum blue

KW - Molybdenum-reducing bacterium

UR - http://www.scopus.com/inward/record.url?scp=85020483126&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020483126&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85020483126

VL - 20

SP - 111

EP - 134

JO - Malaysian Journal of Soil Science

JF - Malaysian Journal of Soil Science

SN - 1394-7990

ER -