A mediator-less microbial fuel cell using a metal reducing bacterium, Shewanella putrefaciens

Hyung Joo Kim, Hyung Soo Park, Moon Sik Hyun, In Seop Chang, Mia Kim, Byung Hong Kim

Research output: Contribution to journalArticle

668 Citations (Scopus)

Abstract

Direct electron transfer from different Shewanella putrefaciens strains to an electrode was examined using cyclic voltammetry and a fuel cell type electrochemical cell. Both methods determine the electrochemical activity of the bacterium without any electrochemical mediators. In the cyclic voltammetric studies, anaerobically grown cells of Shewanella putrefaciens MR-1, IR-1, and SR-21 showed electrochemical activities, but no activities were observed in aerobically grown Shewanella putrefaciens cells nor in aerobically and anaerobically grown E. coli cell suspensions. The electrochemical activities measured by the cyclic voltammetric method were closely related to the electric potential and current generation capacities in the microbial fuel cell system. Cytochromes localized to the outer membrane are believed to facilitate the direct electron transfer to the electrode from the intact bacterial cells. The concentration of the electron donor in the anode compartment determined the current generation capacity and potential development in the microbial fuel cell. When the high concentration of the bacteria (0.47 g dry cell weight/liter) and an electrode that has large surface area (apparent area: 50 cm2) were used, relatively high Coulombic yield (over 3 C for 12 h) was obtained from the bacteria.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalEnzyme and Microbial Technology
Volume30
Issue number2
DOIs
Publication statusPublished - 14 Feb 2002
Externally publishedYes

Fingerprint

Shewanella putrefaciens
Bioelectric Energy Sources
Microbial fuel cells
Bacteria
Metals
Electrodes
Electrons
Electrochemical cells
Electric currents
Cytochromes
Escherichia coli
Cyclic voltammetry
Fuel cells
Suspensions
Anodes
Proteins
Membranes
Electric potential
Weights and Measures

Keywords

  • Mediator-less microbial fuel cell
  • Metal-reducing bacteria
  • Shewanella purefaciens

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

A mediator-less microbial fuel cell using a metal reducing bacterium, Shewanella putrefaciens. / Kim, Hyung Joo; Park, Hyung Soo; Hyun, Moon Sik; Chang, In Seop; Kim, Mia; Kim, Byung Hong.

In: Enzyme and Microbial Technology, Vol. 30, No. 2, 14.02.2002, p. 145-152.

Research output: Contribution to journalArticle

Kim, Hyung Joo ; Park, Hyung Soo ; Hyun, Moon Sik ; Chang, In Seop ; Kim, Mia ; Kim, Byung Hong. / A mediator-less microbial fuel cell using a metal reducing bacterium, Shewanella putrefaciens. In: Enzyme and Microbial Technology. 2002 ; Vol. 30, No. 2. pp. 145-152.
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