A lithotrophic microbial fuel cell operated with pseudomonads-dominated iron-oxidizing bacteria enriched at the anode

Thuy Thu Nguyen, Tha Thanh Thi Luong, Phuong Hoang Nguyen Tran, Ha Thi Viet Bui, Huy Quang Nguyen, Hang Thuy Dinh, Byung Hong Kim, Hai The Pham

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    In this study, we attempted to enrich neutrophilic iron bacteria in a microbial fuel cell (MFC)-type reactor in order to develop a lithotrophic MFC system that can utilize ferrous iron as an inorganic electron donor and operate at neutral pHs. Electrical currents were steadily generated at an average level of 0.6mA (or 0.024mA cm<sup>-2</sup> of membrane area) in reactors initially inoculated with microbial sources and operated with 20mM Fe<sup>2+</sup> as the sole electron donor and 10ohm external resistance; whereas in an uninoculated reactor (the control), the average current level only reached 0.2mA (or 0.008mAcm<sup>-2</sup> of membrane area). In an inoculated MFC, the generation of electrical currents was correlated with increases in cell density of bacteria in the anode suspension and coupled with the oxidation of ferrous iron. Cultivation-based and denaturing gradient gel electrophoresis analyses both show the dominance of some Pseudomonas species in the anode communities of the MFCs. Fluorescent in-situ hybridization results revealed significant increases of neutrophilic iron-oxidizing bacteria in the anode community of an inoculated MFC. The results, altogether, prove the successful development of a lithotrophic MFC system with iron bacteria enriched at its anode and suggest a chemolithotrophic anode reaction involving some Pseudomonas species as key players in such a system. The system potentially offers unique applications, such as accelerated bioremediation or on-site biodetection of iron and/or manganese in water samples. In this study, we attempted to enrich neutrophilic iron bacteria in a microbial fuel cell (MFC) typed reactor in order to develop a lithotrophic MFC system that can utilize ferrous iron as an inorganic electron donor and operate at neutral pHs. Our results proved the successful development of such a lithotrophic MFC system with iron bacteria enriched at its anode and suggest a chemolithotrophic anode reaction involving some Pseudomonas species as key players in such a system. The system potentially offers unique applications, such as accelerated bioremediation or on-site bio-detection of iron and/or manganese in water samples.

    Original languageEnglish
    Pages (from-to)579-589
    Number of pages11
    JournalMicrobial Biotechnology
    Volume8
    Issue number3
    DOIs
    Publication statusPublished - 1 May 2015

    Fingerprint

    Bioelectric Energy Sources
    Microbial fuel cells
    Bacteria
    Anodes
    Electrodes
    Iron
    Pseudomonas
    Environmental Biodegradation
    Bioremediation
    Electrons
    Manganese
    Membranes
    Denaturing Gradient Gel Electrophoresis
    Water
    Electrophoresis
    Fluorescence In Situ Hybridization
    Suspensions
    Gels
    Cell Count

    ASJC Scopus subject areas

    • Applied Microbiology and Biotechnology
    • Biotechnology
    • Biochemistry
    • Bioengineering

    Cite this

    Nguyen, T. T., Luong, T. T. T., Tran, P. H. N., Bui, H. T. V., Nguyen, H. Q., Dinh, H. T., ... Pham, H. T. (2015). A lithotrophic microbial fuel cell operated with pseudomonads-dominated iron-oxidizing bacteria enriched at the anode. Microbial Biotechnology, 8(3), 579-589. https://doi.org/10.1111/1751-7915.12267

    A lithotrophic microbial fuel cell operated with pseudomonads-dominated iron-oxidizing bacteria enriched at the anode. / Nguyen, Thuy Thu; Luong, Tha Thanh Thi; Tran, Phuong Hoang Nguyen; Bui, Ha Thi Viet; Nguyen, Huy Quang; Dinh, Hang Thuy; Kim, Byung Hong; Pham, Hai The.

    In: Microbial Biotechnology, Vol. 8, No. 3, 01.05.2015, p. 579-589.

    Research output: Contribution to journalArticle

    Nguyen, TT, Luong, TTT, Tran, PHN, Bui, HTV, Nguyen, HQ, Dinh, HT, Kim, BH & Pham, HT 2015, 'A lithotrophic microbial fuel cell operated with pseudomonads-dominated iron-oxidizing bacteria enriched at the anode', Microbial Biotechnology, vol. 8, no. 3, pp. 579-589. https://doi.org/10.1111/1751-7915.12267
    Nguyen, Thuy Thu ; Luong, Tha Thanh Thi ; Tran, Phuong Hoang Nguyen ; Bui, Ha Thi Viet ; Nguyen, Huy Quang ; Dinh, Hang Thuy ; Kim, Byung Hong ; Pham, Hai The. / A lithotrophic microbial fuel cell operated with pseudomonads-dominated iron-oxidizing bacteria enriched at the anode. In: Microbial Biotechnology. 2015 ; Vol. 8, No. 3. pp. 579-589.
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