A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres

Eda Yuhana Ariffin, Nik Nurhanan Nik Mansor, Eka Safitri, Yook Heng Lee, Nurul Izzaty Hassan

Research output: Contribution to journalArticle

Abstract

The sensitive and rapid detection of hydrogen peroxide is very important in the areas of clinical and environmental analyses. A sensitive and selective Horseradish peroxidase (HRP)-hydrogen peroxide (H2O2) biosensor was developed based on acrylic microspheres. Hydrophobic poly(n-butyl acrylate-N-acryloxysuccinimide) [poly(nBA-NAS)] microspheres were synthesized using photopolymerization in an emulsion to form an enzyme immobilization matrix. The HRP enzyme was covalently immobilized onto the acrylic microspheres via the succinimide functionality. Field emission scanning electron microscope (FESEM) has been utilized to characterize the screen-printed carbon paste electrode (SPE) constructed from enzyme conjugated acrylic microspheres and gold nanoparticles (AuNPs) composite (HRP/nBA-NAS/AuNPs/SPE). Differential pulse voltammetry was used to assess the biosensor performance. The linear response range of the hydrogen peroxide biosensor obtained was from 1.0 × 10-2 to 1.0 × 10-10 M (R2 = 0.99) with the limit of detection (LOD) approximately at 1.0 × 10-10 M. This is an improvement over many hydrogen peroxide biosensors reported so far. Such improvement may be attributed to the large surface area provided by the acrylic microspheres as a matrix for immobilization of the HRP enzyme.

Original languageEnglish
Pages (from-to)1409-1416
Number of pages8
JournalSains Malaysiana
Volume48
Issue number7
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Horseradish Peroxidase
Microspheres
Biosensors
Hydrogen Peroxide
Acrylics
Ointments
Enzymes
Carbon
Enzyme immobilization
Electrodes
Photopolymerization
Voltammetry
Emulsions
Gold
Field emission
Electron microscopes
Nanoparticles
Scanning
Composite materials

Keywords

  • Acrylic microsphere
  • Biosensor
  • Horseradish peroxidase
  • Hydrogen peroxide

ASJC Scopus subject areas

  • General

Cite this

A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres. / Ariffin, Eda Yuhana; Nik Mansor, Nik Nurhanan; Safitri, Eka; Lee, Yook Heng; Hassan, Nurul Izzaty.

In: Sains Malaysiana, Vol. 48, No. 7, 01.01.2019, p. 1409-1416.

Research output: Contribution to journalArticle

Ariffin, Eda Yuhana ; Nik Mansor, Nik Nurhanan ; Safitri, Eka ; Lee, Yook Heng ; Hassan, Nurul Izzaty. / A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres. In: Sains Malaysiana. 2019 ; Vol. 48, No. 7. pp. 1409-1416.
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