A Highly Sensitive Electrochemical DNA Biosensor from Acrylic-Gold Nano-composite for the Determination of Arowana Fish Gender

Mahbubur Rahman, Yook Heng Lee, Dedi Futra, Chew Poh Chiang, Zulkafli A. Rashid, Ling Ling Tan @ Chong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The present research describes a simple method for the identification of the gender of arowana fish (Scleropages formosus). The DNA biosensor was able to detect specific DNA sequence at extremely low level down to atto M regimes. An electrochemical DNA biosensor based on acrylic microsphere-gold nanoparticle (AcMP-AuNP) hybrid composite was fabricated. Hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesised with a facile and well-established one-step photopolymerization procedure and physically adsorbed on the AuNPs at the surface of a carbon screen printed electrode (SPE). The DNA biosensor was constructed simply by grafting an aminated DNA probe on the succinimide functionalised AcMPs via a strong covalent attachment. DNA hybridisation response was determined by differential pulse voltammetry (DPV) technique using anthraquinone monosulphonic acid redox probe as an electroactive oligonucleotide label (Table 1). A low detection limit at 1.0 × 10−18 M with a wide linear calibration range of 1.0 × 10−18 to 1.0 × 10−8 M (R2 = 0.99) can be achieved by the proposed DNA biosensor under optimal conditions. Electrochemical detection of arowana DNA can be completed within 1 hour. Due to its small size and light weight, the developed DNA biosensor holds high promise for the development of functional kit for fish culture usage.

Original languageEnglish
Article number484
JournalNanoscale Research Letters
Volume12
DOIs
Publication statusPublished - 2017

Fingerprint

fishes
bioinstrumentation
Biosensors
Gold
Fish
Acrylics
DNA
deoxyribonucleic acid
gold
composite materials
Composite materials
Microspheres
Anthraquinones
Photopolymerization
DNA sequences
succinimides
DNA Probes
Voltammetry
hybrid composites
Oligonucleotides

Keywords

  • Acrylic microspheres
  • Arowana DNA
  • DNA biosensor
  • DNA hybridization
  • Electrochemical biosensor
  • Photopolymerization

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

Cite this

A Highly Sensitive Electrochemical DNA Biosensor from Acrylic-Gold Nano-composite for the Determination of Arowana Fish Gender. / Rahman, Mahbubur; Lee, Yook Heng; Futra, Dedi; Chiang, Chew Poh; Rashid, Zulkafli A.; Tan @ Chong, Ling Ling.

In: Nanoscale Research Letters, Vol. 12, 484, 2017.

Research output: Contribution to journalArticle

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