A genome level survey of Burkholderia pseudomallei immunome expressed during human infection

Yu Ching Su, Kiew Lian Wan, Rahmah Mohamed, Sheila Nathan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Burkholderia pseudomallei is the etiological agent of melioidosis, a severe infectious disease of humans and animals. The role of the bacterium's proteins expressed in vivo during human melioidosis continues to remain an enigma. This study's aim was to identify B. pseudomallei target proteins that elicit the humoral immune response in infected humans. A small insert genomic expression library was constructed and immunoscreened to identify peptides that reacted exclusively with melioidosis patients' sera. Sero-positive clones expressing immunogenic peptides were sequenced and annotated, and shown to represent 109 proteins involved in bacterial cell envelope biogenesis, cell motility and secretion, transcription, amino acid, ion and protein metabolism, energy production, DNA repair and unknown hypothetical proteins. Western blot analysis of three randomly selected full-length immunogenic polypeptides with patients' sera verified the findings of the immunome screening. The patients' humoral immune response to the 109 proteins suggests the induction or significant upregulation of these proteins in vivo during human infection and thus may play a role in the pathogenesis of B. pseudomallei. Identification of B. pseudomallei immunogens has shed new light on the elucidation of the bacterium's pathogenesis mechanism and disease severity. These immunogens can be further evaluated as prophylactic and serodiagnostic candidates as well as drug targets.

Original languageEnglish
Pages (from-to)1335-1345
Number of pages11
JournalMicrobes and Infection
Volume10
Issue number12-13
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Burkholderia pseudomallei
Genome
Melioidosis
Infection
Proteins
Humoral Immunity
Peptides
Bacteria
Genomic Library
Surveys and Questionnaires
Serum
DNA Repair
Energy Metabolism
Cell Movement
Communicable Diseases
Up-Regulation
Clone Cells
Western Blotting
Ions
Amino Acids

Keywords

  • Burkholderia pseudomallei
  • Immunogens
  • Melioidosis

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

A genome level survey of Burkholderia pseudomallei immunome expressed during human infection. / Su, Yu Ching; Wan, Kiew Lian; Mohamed, Rahmah; Nathan, Sheila.

In: Microbes and Infection, Vol. 10, No. 12-13, 10.2008, p. 1335-1345.

Research output: Contribution to journalArticle

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