A DNA biosensor based on kappa-carrageenan-polypyrrole-gold nanoparticles composite for gender determination of Arowana fish (Scleropages formosus)

Chakavak Esmaeili, Yook Heng Lee, Chew Poh Chiang, Zulkafli A. Rashid, Eka Safitri, Radha Swathe Priya Malon Marugan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The preparation and characterization of a DNA biosensor based on kappa-carrageenan-polypyrrole-gold nanoparticles (KC-PPy-AuNPs) nano-biocomposite is reported to demonstrate an application for gender classification of Arowana fish. Immobilization of the thiol modified Arowana fish ssDNA probe sequence was successfully carried out via covalent attachment to the gold (Au) on the surface of the nano-biocomposite membrane. Under optimum conditions, the DNA biosensor showed a short immobilization and hybridization time with high sensitivity for monitoring the target DNA sequence. The hybridization with the target DNA was determined by monitoring the redox current of anthraquinone-2-sulfonic acid monohydrate sodium salt (AQMS) using differential pulse voltammetry (DPV). The DNA hybridization response gradually increased with increasing concentrations of target DNA from 5 × 10−18 to 5 × 10−12 M and the limit of detection was below 5 × 10−18 M. The application of the DNA biosensor for gender classification of Arowana fish was demonstrated.

Original languageEnglish
Pages (from-to)616-624
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume242
DOIs
Publication statusPublished - 1 Apr 2017

Fingerprint

Carrageenan
polypyrroles
Polypyrroles
fishes
bioinstrumentation
Biosensors
Gold
Fish
DNA
deoxyribonucleic acid
gold
Nanoparticles
nanoparticles
composite materials
Composite materials
immobilization
Monitoring
DNA sequences
anthraquinones
Voltammetry

Keywords

  • AQMS redox label
  • Arowana
  • Differential pulse voltammetry (DPV)
  • Electrochemical DNA biosensor
  • Nano biocomposite

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry
  • Electrical and Electronic Engineering

Cite this

A DNA biosensor based on kappa-carrageenan-polypyrrole-gold nanoparticles composite for gender determination of Arowana fish (Scleropages formosus). / Esmaeili, Chakavak; Lee, Yook Heng; Chiang, Chew Poh; Rashid, Zulkafli A.; Safitri, Eka; Malon Marugan, Radha Swathe Priya.

In: Sensors and Actuators, B: Chemical, Vol. 242, 01.04.2017, p. 616-624.

Research output: Contribution to journalArticle

Esmaeili, Chakavak ; Lee, Yook Heng ; Chiang, Chew Poh ; Rashid, Zulkafli A. ; Safitri, Eka ; Malon Marugan, Radha Swathe Priya. / A DNA biosensor based on kappa-carrageenan-polypyrrole-gold nanoparticles composite for gender determination of Arowana fish (Scleropages formosus). In: Sensors and Actuators, B: Chemical. 2017 ; Vol. 242. pp. 616-624.
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