A DNA biosensor based on gold nanoparticle decorated on carboxylated multi-walled carbon nanotubes for gender determination of Arowana fish

Kasra Saeedfar, Yook Heng Lee, Chew Poh Chiang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Multi-wall carbon nanotubes (MWCNTs) were modified to design a new DNA biosensor. Functionalized MWCNTs were equipped with gold nanoparticles (GNPs) (~ 15 nm) (GNP-MWCNTCOOH) to construct DNA biosensors based on carbon-paste screen-printed (SPE) electrodes. GNP attachment onto functionalized MWCNTs was carried out by microwave irradiation and was confirmed by spectroscopic studies and surface analysis. DNA biosensors based on differential pulse voltammetry (DPV) were constructed by immobilizing thiolated single-stranded DNA probes onto GNP-MWCNTCOOH. Ruthenium (III) chloride hexaammoniate [Ru(NH3)6,2Cl] (RuHex) was used as hybridization redox indicator. RuHex and MWCNT interaction was low in compared to other organic redox hybridization indicators. The linear response range for DNA determination was 1 × 10− 21 to 1 × 10− 9 M with a lower detection limit of 1.55 × 10− 21 M. Thus, the attachment of GNPs onto functionalized MWCNTs yielded sensitive DNA biosensor with low detection limit and stability more than 30 days. Constructed electrode was used to determine gender of arowana fish.

Original languageEnglish
Pages (from-to)106-113
Number of pages8
JournalBioelectrochemistry
Volume118
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

Carbon Nanotubes
fishes
Biosensing Techniques
bioinstrumentation
Biosensors
Gold
Nanoparticles
Fish
Carbon nanotubes
Fishes
DNA
deoxyribonucleic acid
carbon nanotubes
gold
nanoparticles
Oxidation-Reduction
Limit of Detection
Electrodes
attachment
Ruthenium

Keywords

  • Arowana fish
  • Carbon nanotube
  • Differential pulse voltammetry
  • DNA biosensor
  • Ruthenium (III) chloride hexaammoniate

ASJC Scopus subject areas

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

Cite this

A DNA biosensor based on gold nanoparticle decorated on carboxylated multi-walled carbon nanotubes for gender determination of Arowana fish. / Saeedfar, Kasra; Lee, Yook Heng; Chiang, Chew Poh.

In: Bioelectrochemistry, Vol. 118, 01.12.2017, p. 106-113.

Research output: Contribution to journalArticle

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