A defect in DNA topoisomerase II activity in ataxia-telangiectasia cells

Rahmah Mohamed, Surinder Pal Singh, Sharad Kumar, Martin F. Lavin

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    Abstract

    DNA topoisomerase type I and II activities were determined by serial dilution in nuclear extracts from control and ataxia-telangiectasia lymphoblastoid cells. Topoisomerase I activity, assayed by relaxation of supercoiled plasmid DNA, was found to be approximately the same in both cell types. In order to remove interference from topoisomerase I, the activity of topoisomerase II was measured by the unknotting of knotted P4 phage DNA in the presence of ATP. The activity of topoisomerase II was markedly reduced in two ataxia-telangiectasia cell lines, AT2ABR and AT8ABR, compared to controls. This reduction in activity was detected with increasing concentration of protein and in time course experiments at a single protein concentration. A third cell line, AT3ABR, did not have a detectably lower activity of topoisomerase II when assayed under these conditions. The difference in topoisomerase II activity in the ataxia-telangiectasia cell lines examined may reflect to some extent the heterogeneity observed in this syndrome.

    Original languageEnglish
    Pages (from-to)233-238
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume149
    Issue number1
    DOIs
    Publication statusPublished - 30 Nov 1987

    Fingerprint

    Ataxia Telangiectasia
    Type II DNA Topoisomerase
    Type I DNA Topoisomerase
    Defects
    Cells
    Cell Line
    Superhelical DNA
    Bacteriophages
    DNA
    Dilution
    Proteins
    Plasmids
    Adenosine Triphosphate
    Experiments

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    A defect in DNA topoisomerase II activity in ataxia-telangiectasia cells. / Mohamed, Rahmah; Singh, Surinder Pal; Kumar, Sharad; Lavin, Martin F.

    In: Biochemical and Biophysical Research Communications, Vol. 149, No. 1, 30.11.1987, p. 233-238.

    Research output: Contribution to journalArticle

    Mohamed, Rahmah ; Singh, Surinder Pal ; Kumar, Sharad ; Lavin, Martin F. / A defect in DNA topoisomerase II activity in ataxia-telangiectasia cells. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 149, No. 1. pp. 233-238.
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