A copper toxicity biosensor using immobilized cyanobacteria, Anabaena torulosa

Tay Chia Chay, Salmijah Surif, Yook Heng Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A toxicity biosensor based on immobilized Anabaena torulosa for the determination of copper toxicity was developed. The live cyanobacteria cells were immobilized in a poly(2-hydroxyl ethyl methacrylate) (pHEMA) membrane and interfaced with an oxygen electrode. The analytical device allows the monitoring of cell inhibition due to copper toxicity through the changes of photosynthetic oxygen release. The 50% inhibitory response to Cu was between 0.5-0.7 mg/L Cu depending on the cell age. The linear response range to Cu was from 0.3-1.0 mg/L Cu (R2 = 0.9627). The whole-cell biosensor demonstrated rapid response (<5 mins) and the inhibition of photosynthetic activity was reproducible (BSD = 0-14%).

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalSensor Letters
Volume3
Issue number1
DOIs
Publication statusPublished - Mar 2005

Fingerprint

anabaena
bioinstrumentation
Biosensors
toxicity
Toxicity
Copper
copper
cells
Oxygen
oxygen
Membranes
Electrodes
Monitoring
membranes
electrodes
Cyanobacteria

Keywords

  • Inhibition
  • Metal Toxicity
  • Photosynthesis
  • Poly(hydroxylethyl methacrylate)
  • Whole-Cell Biosensor

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Signal Processing
  • Control and Systems Engineering

Cite this

A copper toxicity biosensor using immobilized cyanobacteria, Anabaena torulosa. / Chay, Tay Chia; Surif, Salmijah; Lee, Yook Heng.

In: Sensor Letters, Vol. 3, No. 1, 03.2005, p. 49-54.

Research output: Contribution to journalArticle

Chay, Tay Chia ; Surif, Salmijah ; Lee, Yook Heng. / A copper toxicity biosensor using immobilized cyanobacteria, Anabaena torulosa. In: Sensor Letters. 2005 ; Vol. 3, No. 1. pp. 49-54.
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