A competitive immunosorbent assay for detection of Pseudomonas pseudomallei exotoxin

G. Ismail, Mohammed Noor Embi, O. Omar, J. C. Allen, C. J. Smith

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The development of monoclonal antibody and enzyme-linked immunosorbent assay (ELISA) techniques has made possible the detection of specific antigens at extremely low concentrations. Diagnosis of recalcitrant diseases such as melioidosis depends upon either early isolation and identification of the causative organism or the identification of a specific marker antigen. Pseudomonas pseudomallei exotoxin, in serum; the latter is better because it allows more rapid and simple diagnosis. A method of detecting exotoxin concentrations of > 16 ng/ml by an ELISA based on a monoclonal antitoxin is here described; it is significantly more sensitive than the mouse lethality test (lower threshold 30 μg/ml) currently in use and in-vitro mouse lethality test (lower threshold 30 μg/ml) currently in use and an in-vitro cytotoxicity test (lower threshold 10 μg/ml) that we have developed and describe here.

Original languageEnglish
Pages (from-to)353-357
Number of pages5
JournalJournal of Medical Microbiology
Volume23
Issue number4
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Burkholderia pseudomallei
Exotoxins
Immunosorbents
Enzyme-Linked Immunosorbent Assay
Immunosorbent Techniques
Melioidosis
Antitoxins
Differentiation Antigens
Monoclonal Antibodies
Antigens
Serum
In Vitro Techniques

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Ismail, G., Embi, M. N., Omar, O., Allen, J. C., & Smith, C. J. (1987). A competitive immunosorbent assay for detection of Pseudomonas pseudomallei exotoxin. Journal of Medical Microbiology, 23(4), 353-357.

A competitive immunosorbent assay for detection of Pseudomonas pseudomallei exotoxin. / Ismail, G.; Embi, Mohammed Noor; Omar, O.; Allen, J. C.; Smith, C. J.

In: Journal of Medical Microbiology, Vol. 23, No. 4, 1987, p. 353-357.

Research output: Contribution to journalArticle

Ismail, G, Embi, MN, Omar, O, Allen, JC & Smith, CJ 1987, 'A competitive immunosorbent assay for detection of Pseudomonas pseudomallei exotoxin', Journal of Medical Microbiology, vol. 23, no. 4, pp. 353-357.
Ismail, G. ; Embi, Mohammed Noor ; Omar, O. ; Allen, J. C. ; Smith, C. J. / A competitive immunosorbent assay for detection of Pseudomonas pseudomallei exotoxin. In: Journal of Medical Microbiology. 1987 ; Vol. 23, No. 4. pp. 353-357.
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