A comparative study on biosurfactant activity of crude oil-degrading bacteria and its correlation to total petroleum hydrocarbon degradation

Chia Wei Phan, Nur Faizah Abu Bakar, Ainon Hamzah

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    In this study, 11 bacteria isolated from Tapis crude oil-contaminated sites were identified by using biochemical tests and 16S rDNA gene sequencing. Their abilities to biodegrade Tapis crude oil was determined by gas chromatography before they were further screened for biosurfactant activity by employing qualitative (blood agar hemolysis, microplate assay, drop-collapse test), semiquantitative (emulsification formation), and quantitative (surface tension measurement) methods. Four isolates, namely, Acinetobacter baumanii UKMP-12T, Pseudomonas aeruginosa UKMP-14T, Rhodococcus sp. UKMP-5T, and Rhodococcus sp. UKMP-7T, exhibited high percentages in total petroleum hydrocarbon (TPH) degradation. A strong correlation between the emulsification index (E 24) and surface tension measurement (r s = +.866) as shown by Spearman rank correlation analysis suggested that these two methods were more reliable to predict biosurfactant activity. The TPH removal was also positively correlated to the ability of bacterial isolates to reduce the surface tension of growth medium, as revealed by Pearson correlation test rp = +.886). In conclusion, not all the biosurfactant detection protocols employed were effective. Nevertheless, the measurement of surface tension and E 24 determination provided a rather rapid, easy, reproducible, and accurate result in identifying bacteria with biosurfactant-producing ability.

    Original languageEnglish
    Pages (from-to)240-251
    Number of pages12
    JournalBioremediation Journal
    Volume17
    Issue number4
    DOIs
    Publication statusPublished - 2 Oct 2013

    Fingerprint

    surface tension
    petroleum hydrocarbon
    crude oil
    comparative study
    degradation
    bacterium
    microplate
    measurement method
    agar
    gas chromatography
    blood
    assay
    gene
    test
    emulsification

    Keywords

    • biodegradation
    • bioremediation
    • biosurfactant-producing bacteria
    • correlation
    • surface tension
    • Tapis crude oil
    • total petroleum hydrocarbon

    ASJC Scopus subject areas

    • Environmental Science(all)

    Cite this

    A comparative study on biosurfactant activity of crude oil-degrading bacteria and its correlation to total petroleum hydrocarbon degradation. / Phan, Chia Wei; Abu Bakar, Nur Faizah; Hamzah, Ainon.

    In: Bioremediation Journal, Vol. 17, No. 4, 02.10.2013, p. 240-251.

    Research output: Contribution to journalArticle

    @article{2a4f15d51c4943a8bb70a2637ca19d07,
    title = "A comparative study on biosurfactant activity of crude oil-degrading bacteria and its correlation to total petroleum hydrocarbon degradation",
    abstract = "In this study, 11 bacteria isolated from Tapis crude oil-contaminated sites were identified by using biochemical tests and 16S rDNA gene sequencing. Their abilities to biodegrade Tapis crude oil was determined by gas chromatography before they were further screened for biosurfactant activity by employing qualitative (blood agar hemolysis, microplate assay, drop-collapse test), semiquantitative (emulsification formation), and quantitative (surface tension measurement) methods. Four isolates, namely, Acinetobacter baumanii UKMP-12T, Pseudomonas aeruginosa UKMP-14T, Rhodococcus sp. UKMP-5T, and Rhodococcus sp. UKMP-7T, exhibited high percentages in total petroleum hydrocarbon (TPH) degradation. A strong correlation between the emulsification index (E 24) and surface tension measurement (r s = +.866) as shown by Spearman rank correlation analysis suggested that these two methods were more reliable to predict biosurfactant activity. The TPH removal was also positively correlated to the ability of bacterial isolates to reduce the surface tension of growth medium, as revealed by Pearson correlation test rp = +.886). In conclusion, not all the biosurfactant detection protocols employed were effective. Nevertheless, the measurement of surface tension and E 24 determination provided a rather rapid, easy, reproducible, and accurate result in identifying bacteria with biosurfactant-producing ability.",
    keywords = "biodegradation, bioremediation, biosurfactant-producing bacteria, correlation, surface tension, Tapis crude oil, total petroleum hydrocarbon",
    author = "Phan, {Chia Wei} and {Abu Bakar}, {Nur Faizah} and Ainon Hamzah",
    year = "2013",
    month = "10",
    day = "2",
    doi = "10.1080/10889868.2013.827621",
    language = "English",
    volume = "17",
    pages = "240--251",
    journal = "Bioremediation Journal",
    issn = "1088-9868",
    publisher = "Taylor and Francis Ltd.",
    number = "4",

    }

    TY - JOUR

    T1 - A comparative study on biosurfactant activity of crude oil-degrading bacteria and its correlation to total petroleum hydrocarbon degradation

    AU - Phan, Chia Wei

    AU - Abu Bakar, Nur Faizah

    AU - Hamzah, Ainon

    PY - 2013/10/2

    Y1 - 2013/10/2

    N2 - In this study, 11 bacteria isolated from Tapis crude oil-contaminated sites were identified by using biochemical tests and 16S rDNA gene sequencing. Their abilities to biodegrade Tapis crude oil was determined by gas chromatography before they were further screened for biosurfactant activity by employing qualitative (blood agar hemolysis, microplate assay, drop-collapse test), semiquantitative (emulsification formation), and quantitative (surface tension measurement) methods. Four isolates, namely, Acinetobacter baumanii UKMP-12T, Pseudomonas aeruginosa UKMP-14T, Rhodococcus sp. UKMP-5T, and Rhodococcus sp. UKMP-7T, exhibited high percentages in total petroleum hydrocarbon (TPH) degradation. A strong correlation between the emulsification index (E 24) and surface tension measurement (r s = +.866) as shown by Spearman rank correlation analysis suggested that these two methods were more reliable to predict biosurfactant activity. The TPH removal was also positively correlated to the ability of bacterial isolates to reduce the surface tension of growth medium, as revealed by Pearson correlation test rp = +.886). In conclusion, not all the biosurfactant detection protocols employed were effective. Nevertheless, the measurement of surface tension and E 24 determination provided a rather rapid, easy, reproducible, and accurate result in identifying bacteria with biosurfactant-producing ability.

    AB - In this study, 11 bacteria isolated from Tapis crude oil-contaminated sites were identified by using biochemical tests and 16S rDNA gene sequencing. Their abilities to biodegrade Tapis crude oil was determined by gas chromatography before they were further screened for biosurfactant activity by employing qualitative (blood agar hemolysis, microplate assay, drop-collapse test), semiquantitative (emulsification formation), and quantitative (surface tension measurement) methods. Four isolates, namely, Acinetobacter baumanii UKMP-12T, Pseudomonas aeruginosa UKMP-14T, Rhodococcus sp. UKMP-5T, and Rhodococcus sp. UKMP-7T, exhibited high percentages in total petroleum hydrocarbon (TPH) degradation. A strong correlation between the emulsification index (E 24) and surface tension measurement (r s = +.866) as shown by Spearman rank correlation analysis suggested that these two methods were more reliable to predict biosurfactant activity. The TPH removal was also positively correlated to the ability of bacterial isolates to reduce the surface tension of growth medium, as revealed by Pearson correlation test rp = +.886). In conclusion, not all the biosurfactant detection protocols employed were effective. Nevertheless, the measurement of surface tension and E 24 determination provided a rather rapid, easy, reproducible, and accurate result in identifying bacteria with biosurfactant-producing ability.

    KW - biodegradation

    KW - bioremediation

    KW - biosurfactant-producing bacteria

    KW - correlation

    KW - surface tension

    KW - Tapis crude oil

    KW - total petroleum hydrocarbon

    UR - http://www.scopus.com/inward/record.url?scp=84887586037&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84887586037&partnerID=8YFLogxK

    U2 - 10.1080/10889868.2013.827621

    DO - 10.1080/10889868.2013.827621

    M3 - Article

    VL - 17

    SP - 240

    EP - 251

    JO - Bioremediation Journal

    JF - Bioremediation Journal

    SN - 1088-9868

    IS - 4

    ER -