A comparative study of skin cell activities in collagen and fibrin constructs

Jia Xian Law, Faiza Musa, Ruszymah Idrus, Alicia J. El Haj, Ying Yang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Collagen and fibrin are widely used in tissue engineering due to their excellent biocompatibility and bioactivities that support in vivo tissue formation. These two hydrogels naturally present in different wound healing stages with different regulatory effects on cells, and both of them are mechanically weak in the reconstructed hydrogels. We conducted a comparative study by the growth of rat dermal fibroblasts or dermal fibroblasts and epidermal keratinocytes together in collagen and fibrin constructs respectively with and without the reinforcement of electrospun poly(lactic acid) nanofiber mesh. Cell proliferation, gel contraction and elastic modulus of the constructs were measured on the same gels at multiple time points during the 22 day culturing period using multiple non-destructive techniques. The results demonstrated considerably different cellular activities within the two types of constructs. Co-culturing keratinocytes with fibroblasts in the collagen constructs reduced the fibroblast proliferation, collagen contraction and mechanical strength at late culture point regardless of the presence of nanofibers. Co-culturing keratinocytes with fibroblasts in the fibrin constructs promoted fibroblast proliferation but exerted no influence on fibrin contraction and mechanical strength. The presence of nanofibers in the collagen and fibrin constructs played a favorable role on the fibroblast proliferation when keratinocytes were absent. Thus, this study exhibited new evidence of the strong cross-talk between keratinocytes and fibroblasts, which can be used to control fibroblast proliferation and construct contraction. This cross-talk activity is extracellular matrix-dependent in terms of the fibrous network morphology, density and strength.

Original languageEnglish
JournalMedical Engineering and Physics
DOIs
Publication statusAccepted/In press - 1 Sep 2015

Fingerprint

Fibroblasts
Fibrin
Collagen
Skin
Keratinocytes
Nanofibers
Hydrogels
Strength of materials
Gels
Elastic Modulus
Cell proliferation
Lactic acid
Tissue Engineering
Bioactivity
Biocompatibility
Tissue engineering
Wound Healing
Extracellular Matrix
Rats
Reinforcement

Keywords

  • Collagen
  • Contraction
  • Fibrin
  • Fibroblast
  • Keratinocyte
  • Mechanical property
  • Nanofiber
  • Proliferation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biophysics

Cite this

A comparative study of skin cell activities in collagen and fibrin constructs. / Law, Jia Xian; Musa, Faiza; Idrus, Ruszymah; El Haj, Alicia J.; Yang, Ying.

In: Medical Engineering and Physics, 01.09.2015.

Research output: Contribution to journalArticle

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