A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1

Yusuf Muhammad Noor, Nurul Hidayah Samsulrizal, Noor Azah Jema'on, Kheng Oon Low, Aizi Nor Mazila Ramli, Noor Izawati Alias, Siti Intan Rosdianah Damis, Siti Fatimah Zaharah Mohd Fuzi, Mohd Noor Mat Isa, Abdul Munir Abd. Murad, Mohd Firdaus Mohd Raih, Farah Diba Abu Bakar, Nazalan Najimudin, Nor Muhammad Mahadi, Rosli Md Illias

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Bacillus lehensis G1 is a Gram-positive, moderately alkalitolerant bacterium isolated from soil samples. B. lehensis produces cyclodextrin glucanotransferase (CGTase), an enzyme that has enabled the extensive use of cyclodextrin in foodstuffs, chemicals, and pharmaceuticals. The genome sequence of B. lehensis G1 consists of a single circular 3.99. Mb chromosome containing 4017 protein-coding sequences (CDSs), of which 2818 (70.15%) have assigned biological roles, 936 (23.30%) have conserved domains with unknown functions, and 263 (6.55%) have no match with any protein database. Bacillus clausii KSM-K16 was established as the closest relative to B. lehensis G1 based on gene content similarity and 16S rRNA phylogenetic analysis. A total of 2820 proteins from B. lehensis G1 were found to have orthologues in B. clausii, including sodium-proton antiporters, transport proteins, and proteins involved in ATP synthesis. A comparative analysis of these proteins and those in B. clausii and other alkaliphilic Bacillus species was carried out to investigate their contributions towards the alkalitolerance of the microorganism. The similarities and differences in alkalitolerance-related genes among alkalitolerant/alkaliphilic Bacillus species highlight the complex mechanism of pH homeostasis. The B. lehensis G1 genome was also mined for proteins and enzymes with potential viability for industrial and commercial purposes.

Original languageEnglish
Pages (from-to)253-261
Number of pages9
JournalGene
Volume545
Issue number2
DOIs
Publication statusPublished - 25 Jul 2014

Fingerprint

Bacillus
Soil
Bacteria
Proteins
Genome
Protein Databases
Sodium-Hydrogen Antiporter
Cyclodextrins
Enzymes
Genes
Carrier Proteins
Homeostasis
Chromosomes
Adenosine Triphosphate
Pharmaceutical Preparations
Bacillus clausii

Keywords

  • Alkaliphile
  • Alkalitolerant
  • Bacillus lehensis
  • Biotechnological potential
  • Whole-genome sequencing

ASJC Scopus subject areas

  • Genetics

Cite this

Noor, Y. M., Samsulrizal, N. H., Jema'on, N. A., Low, K. O., Ramli, A. N. M., Alias, N. I., ... Illias, R. M. (2014). A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1. Gene, 545(2), 253-261. https://doi.org/10.1016/j.gene.2014.05.012

A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1. / Noor, Yusuf Muhammad; Samsulrizal, Nurul Hidayah; Jema'on, Noor Azah; Low, Kheng Oon; Ramli, Aizi Nor Mazila; Alias, Noor Izawati; Damis, Siti Intan Rosdianah; Fuzi, Siti Fatimah Zaharah Mohd; Isa, Mohd Noor Mat; Abd. Murad, Abdul Munir; Mohd Raih, Mohd Firdaus; Abu Bakar, Farah Diba; Najimudin, Nazalan; Mahadi, Nor Muhammad; Illias, Rosli Md.

In: Gene, Vol. 545, No. 2, 25.07.2014, p. 253-261.

Research output: Contribution to journalArticle

Noor, YM, Samsulrizal, NH, Jema'on, NA, Low, KO, Ramli, ANM, Alias, NI, Damis, SIR, Fuzi, SFZM, Isa, MNM, Abd. Murad, AM, Mohd Raih, MF, Abu Bakar, FD, Najimudin, N, Mahadi, NM & Illias, RM 2014, 'A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1', Gene, vol. 545, no. 2, pp. 253-261. https://doi.org/10.1016/j.gene.2014.05.012
Noor YM, Samsulrizal NH, Jema'on NA, Low KO, Ramli ANM, Alias NI et al. A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1. Gene. 2014 Jul 25;545(2):253-261. https://doi.org/10.1016/j.gene.2014.05.012
Noor, Yusuf Muhammad ; Samsulrizal, Nurul Hidayah ; Jema'on, Noor Azah ; Low, Kheng Oon ; Ramli, Aizi Nor Mazila ; Alias, Noor Izawati ; Damis, Siti Intan Rosdianah ; Fuzi, Siti Fatimah Zaharah Mohd ; Isa, Mohd Noor Mat ; Abd. Murad, Abdul Munir ; Mohd Raih, Mohd Firdaus ; Abu Bakar, Farah Diba ; Najimudin, Nazalan ; Mahadi, Nor Muhammad ; Illias, Rosli Md. / A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1. In: Gene. 2014 ; Vol. 545, No. 2. pp. 253-261.
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