A cell-based potentiometric biosensor using the fungus Lentinus sajor-caju for permethrin determination in treated wood

Mohamad Nasir Mat Arip, Yook Heng Lee, Musa Ahmad, Salmiah Ujang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The characteristics of a potentiometric biosensor for the determination of permethrin in treated wood based on immobilised cells of the fungus Lentinus sajor-caju on a potentiometric transducer are reported this paper. The potentiometric biosensor was prepared by immobilisation of the fungus in alginate gel deposited on a pH-sensitive transducer employing a photocurable acrylic matrix. The biosensor gave a good response in detecting permethrin over the range of 1.0-100.0 μM. The slope of the calibration curve was 56.10 mV/decade with detection limit of 1.00 μM. The relative standard deviation for the sensor reproducibility was 4.86%. The response time of the sensor was 5 min at optimum pH 8.0 with 1.00 mg/electrode of fungus L. sajor-caju. The permethrin biosensor performance was compared with the conventional method for permethrin analysis using high performance liquid chromatography (HPLC), and the analytical results agreed well with the HPLC method (at 95% confidence limit). There was no interference from commonly used organophosphorus pesticides such as diazinon, parathion, paraoxon, and methyl parathion.

Original languageEnglish
Pages (from-to)776-781
Number of pages6
JournalTalanta
Volume116
DOIs
Publication statusPublished - 2013

Fingerprint

Lentinula
Permethrin
Biosensing Techniques
Fungi
Biosensors
Wood
High performance liquid chromatography
Transducers
High Pressure Liquid Chromatography
Methyl Parathion
Diazinon
Paraoxon
Parathion
Immobilized Cells
Sensors
Pesticides
Immobilization
Calibration
Reaction Time
Acrylics

Keywords

  • Alginate
  • Lentinus sajor-caju
  • Permethrin
  • Wood preservative analysis

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

A cell-based potentiometric biosensor using the fungus Lentinus sajor-caju for permethrin determination in treated wood. / Arip, Mohamad Nasir Mat; Lee, Yook Heng; Ahmad, Musa; Ujang, Salmiah.

In: Talanta, Vol. 116, 2013, p. 776-781.

Research output: Contribution to journalArticle

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