A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A

Abimael Cruz-Migoni, Guillaume M. Hautbergue, Peter J. Artymiuk, Patrick J. Baker, Monika Bokori-Brown, Chung Te Chang, Mark J. Dickman, Angela Essex-Lopresti, Sarah V. Harding, Nor Muhammad Mahadi, Laura E. Marshall, George W. Mobbs, Rahmah Mohamed, Sheila Nathan, Sarah A. Ngugi, Catherine Ong, Wen Fong Ooi, Lynda J. Partridge, Helen L. Phillips, Mohd Firdaus Mohd RaihSergei Ruzheinikov, Mitali Sarkar-Tyson, Svetlana E. Sedelnikova, Sophie J. Smither, Patrick Tan, Richard W. Titball, Stuart A. Wilson, David W. Rice

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.

Original languageEnglish
Pages (from-to)821-824
Number of pages4
JournalScience
Volume334
Issue number6057
DOIs
Publication statusPublished - 11 Nov 2011

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Burkholderia pseudomallei
Burkholderia
Peptide Initiation Factors
Cytotoxins
Eukaryotic Cells
Glutamine
Names
Virulence
Escherichia coli
Proteins
cytotoxic necrotizing factor type 1

ASJC Scopus subject areas

  • General

Cite this

Cruz-Migoni, A., Hautbergue, G. M., Artymiuk, P. J., Baker, P. J., Bokori-Brown, M., Chang, C. T., ... Rice, D. W. (2011). A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. Science, 334(6057), 821-824. https://doi.org/10.1126/science.1211915

A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. / Cruz-Migoni, Abimael; Hautbergue, Guillaume M.; Artymiuk, Peter J.; Baker, Patrick J.; Bokori-Brown, Monika; Chang, Chung Te; Dickman, Mark J.; Essex-Lopresti, Angela; Harding, Sarah V.; Mahadi, Nor Muhammad; Marshall, Laura E.; Mobbs, George W.; Mohamed, Rahmah; Nathan, Sheila; Ngugi, Sarah A.; Ong, Catherine; Ooi, Wen Fong; Partridge, Lynda J.; Phillips, Helen L.; Mohd Raih, Mohd Firdaus; Ruzheinikov, Sergei; Sarkar-Tyson, Mitali; Sedelnikova, Svetlana E.; Smither, Sophie J.; Tan, Patrick; Titball, Richard W.; Wilson, Stuart A.; Rice, David W.

In: Science, Vol. 334, No. 6057, 11.11.2011, p. 821-824.

Research output: Contribution to journalArticle

Cruz-Migoni, A, Hautbergue, GM, Artymiuk, PJ, Baker, PJ, Bokori-Brown, M, Chang, CT, Dickman, MJ, Essex-Lopresti, A, Harding, SV, Mahadi, NM, Marshall, LE, Mobbs, GW, Mohamed, R, Nathan, S, Ngugi, SA, Ong, C, Ooi, WF, Partridge, LJ, Phillips, HL, Mohd Raih, MF, Ruzheinikov, S, Sarkar-Tyson, M, Sedelnikova, SE, Smither, SJ, Tan, P, Titball, RW, Wilson, SA & Rice, DW 2011, 'A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A', Science, vol. 334, no. 6057, pp. 821-824. https://doi.org/10.1126/science.1211915
Cruz-Migoni A, Hautbergue GM, Artymiuk PJ, Baker PJ, Bokori-Brown M, Chang CT et al. A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. Science. 2011 Nov 11;334(6057):821-824. https://doi.org/10.1126/science.1211915
Cruz-Migoni, Abimael ; Hautbergue, Guillaume M. ; Artymiuk, Peter J. ; Baker, Patrick J. ; Bokori-Brown, Monika ; Chang, Chung Te ; Dickman, Mark J. ; Essex-Lopresti, Angela ; Harding, Sarah V. ; Mahadi, Nor Muhammad ; Marshall, Laura E. ; Mobbs, George W. ; Mohamed, Rahmah ; Nathan, Sheila ; Ngugi, Sarah A. ; Ong, Catherine ; Ooi, Wen Fong ; Partridge, Lynda J. ; Phillips, Helen L. ; Mohd Raih, Mohd Firdaus ; Ruzheinikov, Sergei ; Sarkar-Tyson, Mitali ; Sedelnikova, Svetlana E. ; Smither, Sophie J. ; Tan, Patrick ; Titball, Richard W. ; Wilson, Stuart A. ; Rice, David W. / A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. In: Science. 2011 ; Vol. 334, No. 6057. pp. 821-824.
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