A biosensor for urea from succinimide-modified acrylic microspheres based on reflectance transduction

Alizar Ulianas, Yook Heng Lee, Musa Ahmad

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

New acrylic microspheres were synthesised by photopolymerisation where the succinimide functional group was incorporated during the microsphere preparation. An optical biosensor for urea based on reflectance transduction with a large linear response range to urea was successfully developed using this material. The biosensor utilized succinimide-modified acrylic microspheres immobilized with a Nile blue chromoionophore (ETH 5294) for optical detection and urease enzyme was immobilized on the surface of the microspheres via the succinimide groups. No leaching of the enzyme or chromoionophore was observed. Hydrolysis of the urea by urease changes the pH and leads to a color change of the immobilized chromoionophore. When the color change was monitored by reflectance spectrophotometry, the linear response range of the biosensor to urea was from 0.01 to 1,000 mM (R2 = 0.97) with a limit of detection of 9.97 μM. The biosensor response showed good reproducibility (relative standard deviation = 1.43%, n = 5) with no interference by major cations such as Na+, K+, NH4 + and Mg2+. The use of reflectance as a transduction method led to a large linear response range that is better than that of many urea biosensors based on other optical transduction methods.

Original languageEnglish
Pages (from-to)8323-8338
Number of pages16
JournalSensors
Volume11
Issue number9
DOIs
Publication statusPublished - Sep 2011

Fingerprint

succinimides
Biosensing Techniques
Microspheres
ureas
bioinstrumentation
Biosensors
Urea
Acrylics
reflectance
Urease
enzymes
Enzymes
Color
color
Immobilized Enzymes
Photopolymerization
Spectrophotometry
leaching
spectrophotometry
Functional groups

Keywords

  • Acrylic microspheres
  • Optical biosensor
  • Photopolymerisation
  • Reflectance
  • Succinimide
  • Urea

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Analytical Chemistry
  • Biochemistry

Cite this

A biosensor for urea from succinimide-modified acrylic microspheres based on reflectance transduction. / Ulianas, Alizar; Lee, Yook Heng; Ahmad, Musa.

In: Sensors, Vol. 11, No. 9, 09.2011, p. 8323-8338.

Research output: Contribution to journalArticle

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