A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone-2-sulphonic Acid in Reduced Graphene Oxide

Raja Zaidatul Akhmar Raja Jamaluddin, Lee Yook Heng, Ling Ling Tan, Kwok Feng Chong

Research output: Contribution to journalArticle

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Abstract

An electrochemical DNA biosensor for DNA determination of genetically modified (GM) soybean (CaMV 35S target genes) was developed utilizing a new detection concept based on the adsoption of anthraquinone-2-sulphonic acid (AQMS) on the reduced graphene oxide nano-particles (rGO) during DNA hybridization events. The aminated DNA probe for CaMV 35S was immobilized onto poly(n-butyl acrylate) film modified with succinimide functional groups [poly(nBA-NAS)] via peptide covalent bond. Nanosheets of rGO were entrapped in the poly(nBA-NAS) film to form a conducting [poly(nBA-NAS)-rGO] film of the DNA biosensor. Besides facilitating the electron transfer reactions, the rGO also functioned as an adsorbent for AQMS. The sensing mechanism of the proposed DNA biosensor involved measuring the oxidation current of the AQMS adsorbed on the electrode surface at −0.50 V using differential pulse voltammetry (DPV) before and after a DNA hybridization event. Under optimum conditions, the DNA biosensor demonstrated a linear proportionality between AQMS oxidation signal and logarithm cDNA concentration from 1.0×10−15 M to 1.0×10−8 M target DNA with a detection limit of 6.3×10−16 M. The electrochemical DNA biosensor possessed good selectivity and a shelf life of about 40 days with relative standard deviation of reproducibility obtained in the range of 3.7–4.6% (n=5). Evaluation of the DNA biosensor using GM soybean DNA extracts showed excellent recovery percentages of 97.2–104.0.

LanguageEnglish
Pages250-258
Number of pages9
JournalElectroanalysis
Volume30
Issue number2
DOIs
Publication statusPublished - 1 Feb 2018

Fingerprint

Graphite
Biosensors
Oxides
Graphene
DNA
Adsorption
Acids
anthraquinone sulfonate
Oxidation
Covalent bonds
Nanosheets
DNA Probes
Voltammetry
Adsorbents
Functional groups
Peptides
Complementary DNA
Genes

Keywords

  • AQMS
  • DPV
  • GMO biosensor
  • Graphene
  • Poly(n-butyl acrylate)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone-2-sulphonic Acid in Reduced Graphene Oxide. / Jamaluddin, Raja Zaidatul Akhmar Raja; Heng, Lee Yook; Tan, Ling Ling; Chong, Kwok Feng.

In: Electroanalysis, Vol. 30, No. 2, 01.02.2018, p. 250-258.

Research output: Contribution to journalArticle

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abstract = "An electrochemical DNA biosensor for DNA determination of genetically modified (GM) soybean (CaMV 35S target genes) was developed utilizing a new detection concept based on the adsoption of anthraquinone-2-sulphonic acid (AQMS) on the reduced graphene oxide nano-particles (rGO) during DNA hybridization events. The aminated DNA probe for CaMV 35S was immobilized onto poly(n-butyl acrylate) film modified with succinimide functional groups [poly(nBA-NAS)] via peptide covalent bond. Nanosheets of rGO were entrapped in the poly(nBA-NAS) film to form a conducting [poly(nBA-NAS)-rGO] film of the DNA biosensor. Besides facilitating the electron transfer reactions, the rGO also functioned as an adsorbent for AQMS. The sensing mechanism of the proposed DNA biosensor involved measuring the oxidation current of the AQMS adsorbed on the electrode surface at −0.50 V using differential pulse voltammetry (DPV) before and after a DNA hybridization event. Under optimum conditions, the DNA biosensor demonstrated a linear proportionality between AQMS oxidation signal and logarithm cDNA concentration from 1.0×10−15 M to 1.0×10−8 M target DNA with a detection limit of 6.3×10−16 M. The electrochemical DNA biosensor possessed good selectivity and a shelf life of about 40 days with relative standard deviation of reproducibility obtained in the range of 3.7–4.6{\%} (n=5). Evaluation of the DNA biosensor using GM soybean DNA extracts showed excellent recovery percentages of 97.2–104.0.",
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