5-azacytidine is insufficient for cardiogenesis in human adipose-derived stem cells

Wan Kamarul Zaman Wan Safwani, Suzana Makpol, Somasundaram Sathapan, Chua Kien Hui

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Adipose tissue is a source of multipotent adult stem cells and it has the ability to differentiate into several types of cell lineages such as neuron cells, osteogenic cells and adipogenic cells. Several reports have shown adipose-derived stem cells (ASCs) have the ability to undergo cardiomyogenesis. Studies have shown 5-azacytidine can successfully drive stem cells such as bone marrow derived stem cells to differentiate into cardiomyogenic cells. Therefore, in this study, we investigated the effect 5-azacytidine on the cardiogenic ability of ASCs. Methods. The cardiogenic potential of ASCs was analysed by studying the morphological changes after induction, the changes in the cardiogenic genes expression i.e. GATA4, MLC-2v, MLC-2a, NKX2.5, -MHC, -MHC, Atrial natriuretic peptide (ANP), Connexin 43, Cardiac Troponin C, Cardiac Troponin I and myocyte enhancer factor (MEF2C) and the changes of embryonic stem cells genes expression at P5 and P10 using quantitative PCR. Results: Our results showed that the induced ASCs did not show significant morphological difference compared to the non-induced ASCs. While quantitative PCR data indicated that most cardiogenic genes and stemness genes expression level decreased after induction at P5 and P10. Conclusion: 5-azacytidine is insufficient for the cardiogenic induction of the ASCs.

Original languageEnglish
Article number3
JournalJournal of Negative Results in BioMedicine
Volume11
Issue number1
DOIs
Publication statusPublished - 2012

Fingerprint

Azacitidine
Stem cells
Stem Cells
Gene expression
Gene Expression
Multipotent Stem Cells
Troponin C
Polymerase Chain Reaction
Connexin 43
Adult Stem Cells
Troponin I
Atrial Natriuretic Factor
Cell Lineage
Embryonic Stem Cells
Muscle Cells
Adipose Tissue
Bone Marrow
Neurons
Bone
Genes

Keywords

  • 5-Azacytidine
  • Adipose
  • Cardiogenic
  • Differentiation
  • Heart
  • Ischemia
  • Stem Cells

ASJC Scopus subject areas

  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

5-azacytidine is insufficient for cardiogenesis in human adipose-derived stem cells. / Wan Safwani, Wan Kamarul Zaman; Makpol, Suzana; Sathapan, Somasundaram; Kien Hui, Chua.

In: Journal of Negative Results in BioMedicine, Vol. 11, No. 1, 3, 2012.

Research output: Contribution to journalArticle

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