4-N-trimethylaminobutyraldehyde dehydrogenase: Purification and characterization of an enzyme from Pseudomonas sp. 13CM

Maizom Hassan, Masahiro Okada, Tsuyoshi Ichiyanagi, Nobuhiro Mori

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

4-N-trimethylaminobutyraldehyde dehydrogenase from Pseudomonas sp. 13CM was purified 14-fold to apparent homogeneity by hydrophobic chromatography on a Phenyl-Toyopearl, and affinity chromatography was done on a 5′-AMP Sepharose4B in the presence of dithiothreitol. The enzyme was found to be a trimer with identical 55 kDa subunits. The isoeletric point was found to be 5.5. The optimum temperature and pH were 40 °C and pH 10.0. The purified enzyme was further characterized with respect to substrate specificity, kinetic parameters, and analog inhibition. The Km values for 4-N-trimethylaminobutyraldehyde, 4-dimethylaminobutyraldehyde, and NAD + were 7.4, 51, and 125 μM respectively. The enzyme was inhibited by SH reagents, and by heavy metal ions.

Original languageEnglish
Pages (from-to)155-162
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number1
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Pseudomonas
Purification
Enzymes
Hydrophobic chromatography
enzymes
Affinity chromatography
Heavy Ions
Sulfhydryl Reagents
dithiothreitol
Dithiothreitol
Adenosine Monophosphate
affinity chromatography
substrate specificity
Substrate Specificity
Heavy Metals
Heavy ions
metal ions
Affinity Chromatography
Kinetic parameters
NAD

Keywords

  • Aldehyde dehydrogenase
  • Analog inhibitor
  • Biosynthesis
  • Carnitine
  • Quaternary ammonium compound

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

4-N-trimethylaminobutyraldehyde dehydrogenase : Purification and characterization of an enzyme from Pseudomonas sp. 13CM. / Hassan, Maizom; Okada, Masahiro; Ichiyanagi, Tsuyoshi; Mori, Nobuhiro.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 1, 2008, p. 155-162.

Research output: Contribution to journalArticle

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