2D-PAGE protein analysis of dinoflagellate Alexandrium minutum based on three different temperatures

Norhidayu Abdul Latib, Safida Anira Norshaha, Gires Usup, Nurul Yuziana Mohd Yusof

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Harmful algae bloom or red tide seems to be considered as threat to ecosystem, especially to human consumption because of the production of neurotoxin by dinoflagellates species such as Alexandrium minutum which can lead to paralytic shellfish poisoning. The aim of this study is to determine the most suitable method for protein extraction of A. minutum followed by determination of differential protein expression of A. minutum on three different temperatures (15°C, 26°C and 31.5°C). After the optimization, the protein extract was subjected to two-dimensional polyacrylamide gel electrophoresis (2-DE) to compare the intensity and distribution of the protein spots. Based on quantitative and qualitative protein assessment, use of Trizol reagent is the most suitable method to extract protein from A. minutum. 2-DE analysis of the samples results in different distribution and intensity of the protein spots were compared between 15°C, 26°C and 31.5°C.

Original languageEnglish
Title of host publication2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Electronic)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Other

Other2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/4/1516/4/15

Fingerprint

proteins
temperature
shellfish
red tide
poisoning
ecosystems
algae
tides
electrophoresis
reagents
gels
optimization

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Latib, N. A., Norshaha, S. A., Usup, G., & Mohd Yusof, N. Y. (2015). 2D-PAGE protein analysis of dinoflagellate Alexandrium minutum based on three different temperatures. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium (Vol. 1678). [030027] American Institute of Physics Inc.. https://doi.org/10.1063/1.4931248

2D-PAGE protein analysis of dinoflagellate Alexandrium minutum based on three different temperatures. / Latib, Norhidayu Abdul; Norshaha, Safida Anira; Usup, Gires; Mohd Yusof, Nurul Yuziana.

2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015. 030027.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Latib, NA, Norshaha, SA, Usup, G & Mohd Yusof, NY 2015, 2D-PAGE protein analysis of dinoflagellate Alexandrium minutum based on three different temperatures. in 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. vol. 1678, 030027, American Institute of Physics Inc., 2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015, Selangor, Malaysia, 15/4/15. https://doi.org/10.1063/1.4931248
Latib NA, Norshaha SA, Usup G, Mohd Yusof NY. 2D-PAGE protein analysis of dinoflagellate Alexandrium minutum based on three different temperatures. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678. American Institute of Physics Inc. 2015. 030027 https://doi.org/10.1063/1.4931248
Latib, Norhidayu Abdul ; Norshaha, Safida Anira ; Usup, Gires ; Mohd Yusof, Nurul Yuziana. / 2D-PAGE protein analysis of dinoflagellate Alexandrium minutum based on three different temperatures. 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015.
@inproceedings{c0cc6963ea8d4edeb6d0fe3cbf364d71,
title = "2D-PAGE protein analysis of dinoflagellate Alexandrium minutum based on three different temperatures",
abstract = "Harmful algae bloom or red tide seems to be considered as threat to ecosystem, especially to human consumption because of the production of neurotoxin by dinoflagellates species such as Alexandrium minutum which can lead to paralytic shellfish poisoning. The aim of this study is to determine the most suitable method for protein extraction of A. minutum followed by determination of differential protein expression of A. minutum on three different temperatures (15°C, 26°C and 31.5°C). After the optimization, the protein extract was subjected to two-dimensional polyacrylamide gel electrophoresis (2-DE) to compare the intensity and distribution of the protein spots. Based on quantitative and qualitative protein assessment, use of Trizol reagent is the most suitable method to extract protein from A. minutum. 2-DE analysis of the samples results in different distribution and intensity of the protein spots were compared between 15°C, 26°C and 31.5°C.",
author = "Latib, {Norhidayu Abdul} and Norshaha, {Safida Anira} and Gires Usup and {Mohd Yusof}, {Nurul Yuziana}",
year = "2015",
month = "9",
day = "25",
doi = "10.1063/1.4931248",
language = "English",
volume = "1678",
booktitle = "2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium",
publisher = "American Institute of Physics Inc.",

}

TY - GEN

T1 - 2D-PAGE protein analysis of dinoflagellate Alexandrium minutum based on three different temperatures

AU - Latib, Norhidayu Abdul

AU - Norshaha, Safida Anira

AU - Usup, Gires

AU - Mohd Yusof, Nurul Yuziana

PY - 2015/9/25

Y1 - 2015/9/25

N2 - Harmful algae bloom or red tide seems to be considered as threat to ecosystem, especially to human consumption because of the production of neurotoxin by dinoflagellates species such as Alexandrium minutum which can lead to paralytic shellfish poisoning. The aim of this study is to determine the most suitable method for protein extraction of A. minutum followed by determination of differential protein expression of A. minutum on three different temperatures (15°C, 26°C and 31.5°C). After the optimization, the protein extract was subjected to two-dimensional polyacrylamide gel electrophoresis (2-DE) to compare the intensity and distribution of the protein spots. Based on quantitative and qualitative protein assessment, use of Trizol reagent is the most suitable method to extract protein from A. minutum. 2-DE analysis of the samples results in different distribution and intensity of the protein spots were compared between 15°C, 26°C and 31.5°C.

AB - Harmful algae bloom or red tide seems to be considered as threat to ecosystem, especially to human consumption because of the production of neurotoxin by dinoflagellates species such as Alexandrium minutum which can lead to paralytic shellfish poisoning. The aim of this study is to determine the most suitable method for protein extraction of A. minutum followed by determination of differential protein expression of A. minutum on three different temperatures (15°C, 26°C and 31.5°C). After the optimization, the protein extract was subjected to two-dimensional polyacrylamide gel electrophoresis (2-DE) to compare the intensity and distribution of the protein spots. Based on quantitative and qualitative protein assessment, use of Trizol reagent is the most suitable method to extract protein from A. minutum. 2-DE analysis of the samples results in different distribution and intensity of the protein spots were compared between 15°C, 26°C and 31.5°C.

UR - http://www.scopus.com/inward/record.url?scp=85006230711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85006230711&partnerID=8YFLogxK

U2 - 10.1063/1.4931248

DO - 10.1063/1.4931248

M3 - Conference contribution

VL - 1678

BT - 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium

PB - American Institute of Physics Inc.

ER -