2-deoxyglucose selection system for Agrobacterium-mediated transformation of Solanum lycopersicon and Nicotiana tabacum plants

Abang Masli Dayang Izawati, Mat Yunus Abdul Masani, Ghulam Kadir Ahmad Parveez, Ismanizan Ismail

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An alternative marker to antibiotic and herbicide genes, 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene was used to produce transgenic tomato (Solanum lycopersicon) and tobacco plants (Nicotiana tabacum) via Agrobacterium-mediated transformation approach. A binary vector, pBIDOG with T-DNA cassette consists of CaMV35S-DOGR1-nos was mobilized into Agrobacterium tumefaciens LBA4404 via electroporation and subsequently transformed into tomato and tobacco tissues. Selection of transformants of both plants was carried out by using 600 mg/l 2-deoxyglucose (2-DOG) as selection agent. 2-DOG resistant calli were regenerated into shoots and PCR analysis revealed that 75% and 71% of putative transgenic tomato and tobacco shoots were positive for DOGR1 gene, respectively. Southern blot analysis was carried out to confirm the integration of transgene. The expected ~1.5 kb band was obtained to prove the integration of DOGR1 gene into the plant genome. Transgenic tomato and tobacco plants were successfully regenerated without any phenotypic changes when compared to wild-type plants. Therefore, this alternative selection system can be applied to other plant transformation systems to produce transgenic plants free from antibiotic and herbicide genes, especially for important economic crops. More importantly, this selection system does not lead to any extent hazard to human and animal that subsequently will improve public acceptance of future genetically modified products.

Original languageEnglish
Pages (from-to)813-820
Number of pages8
JournalAustralian Journal of Crop Science
Volume11
Issue number7
DOIs
Publication statusPublished - 2017

Fingerprint

2-deoxyglucose
Solanum lycopersicum
Agrobacterium
Nicotiana tabacum
tobacco
tomatoes
genetically modified organisms
genes
herbicides
antibiotics
shoots
electroporation
Agrobacterium radiobacter
Southern blotting
transgenes
transgenic plants
callus
phosphates
economics
genome

Keywords

  • 2-deoxyglucose
  • Agrobacterium-mediated transformation
  • DOGR1 gene
  • Transgenic tomato and tobacco

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

2-deoxyglucose selection system for Agrobacterium-mediated transformation of Solanum lycopersicon and Nicotiana tabacum plants. / Izawati, Abang Masli Dayang; Masani, Mat Yunus Abdul; Parveez, Ghulam Kadir Ahmad; Ismail, Ismanizan.

In: Australian Journal of Crop Science, Vol. 11, No. 7, 2017, p. 813-820.

Research output: Contribution to journalArticle

Izawati, Abang Masli Dayang ; Masani, Mat Yunus Abdul ; Parveez, Ghulam Kadir Ahmad ; Ismail, Ismanizan. / 2-deoxyglucose selection system for Agrobacterium-mediated transformation of Solanum lycopersicon and Nicotiana tabacum plants. In: Australian Journal of Crop Science. 2017 ; Vol. 11, No. 7. pp. 813-820.
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