γ-tocotrienol prevents oxidative stress-induced telomere shortening in human fibroblasts derived from different aged individuals

Suzana Makpol, Azrina Zainal Abidin, Khalilah Sairin, Musalmah Mazlan, Gapor Md Top, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The effects of palm γ-tocotrienol (GGT) on oxidative stress-induced cellular ageing was investigated in normal human skin fibroblast cell lines derived from different age groups; young (21-year-old, YF), middle (40-year-old, MF) and old (68-year-old, OF). Fibroblast cells were treated with γ-tocotrienol for 24 hours before or after incubation with IC50 dose of H2O2 for 2 hours. Changes in cell viability, telomere length and telomerase activity were assessed using the MTS assay (Promega, USA), Southern blot analysis and telomere repeat amplification protocol respectively. Results showed that treatment with different concentrations of γ-tocotrienol increased fibroblasts viability with optimum dose of 80 μM for YF and 40 μM for both MF and OF. At higher concentrations, γ-tocotrienol treatment caused marked decrease in cell viability with IC50 value of 200 μM (YF), 300 μM (MF) and 100 μM (OF). Exposure to H2O2 decreased cell viability in dose dependent manner, shortened telomere length and reduced telomerase activity in all age groups. The IC50 of H2O2 was found to be; YF (700 μM), MF (400 μM) and OF (100 μM). Results showed that viability increased significantly (p < 0.05) when cells were treated with 80 μM and 40 μM γ-tocotrienol prior or after H2O 2-induced oxidative stress in all age groups. In YF and OF, pretreatment with γ-tocotrienol prevented shortening of telomere length and reduction in telomerase activity. In MF, telomerase activity increased while no changes in telomere length was observed. However, post-treatment of γ-tocotrienol did not exert any significant effects on telomere length and telomerase activity. Thus, these data suggest that γ-tocotrienol protects against oxidative stress-induced cellular ageing by modulating the telomere length possibly via telomerase.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalOxidative Medicine and Cellular Longevity
Volume3
Issue number1
Publication statusPublished - 2010

Fingerprint

Tocotrienols
Telomere Shortening
Oxidative stress
Fibroblasts
Telomerase
Oxidative Stress
Telomere
Cells
Inhibitory Concentration 50
Cell Survival
Age Groups
Cell Aging
Aging of materials
Southern Blotting
Amplification
Assays
Skin
Therapeutics
Cell Line

Keywords

  • γ-tocotrienol
  • Aging
  • Fibroblasts
  • Oxidative stress
  • Telomerase
  • Telomere

ASJC Scopus subject areas

  • Cell Biology
  • Ageing
  • Biochemistry

Cite this

γ-tocotrienol prevents oxidative stress-induced telomere shortening in human fibroblasts derived from different aged individuals. / Makpol, Suzana; Abidin, Azrina Zainal; Sairin, Khalilah; Mazlan, Musalmah; Top, Gapor Md; Ngah, Wan Zurinah Wan.

In: Oxidative Medicine and Cellular Longevity, Vol. 3, No. 1, 2010, p. 35-43.

Research output: Contribution to journalArticle

Makpol, Suzana ; Abidin, Azrina Zainal ; Sairin, Khalilah ; Mazlan, Musalmah ; Top, Gapor Md ; Ngah, Wan Zurinah Wan. / γ-tocotrienol prevents oxidative stress-induced telomere shortening in human fibroblasts derived from different aged individuals. In: Oxidative Medicine and Cellular Longevity. 2010 ; Vol. 3, No. 1. pp. 35-43.
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