γ-Tocotrienol and 6-Gingerol in combination synergistically induce cytotoxicity and apoptosis in HT-29 and SW837 human colorectal cancer cells

Khairunnisa Md Yusof, Suzana Makpol, A. Rahman A. Jamal, Roslan Harun, Norfilza Mohd Mokhtar, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Numerous bioactive compounds have cytotoxic properties towards cancer cells. However, most studies have used single compounds when bioactives may target different pathways and exert greater cytotoxic effects when used in combination. Therefore, the objective of this study was to determine the anti-proliferative effect of γ-tocotrienol (γ-T3) and 6-gingerol (6G) in combination by evaluating apoptosis and active caspase-3 in HT-29 and SW837 colorectal cancer cells. MTS assays were performed to determine the anti-proliferative and cytotoxicity effect of γ-T3 (0-150 μg/mL) and 6G (0-300 μg/mL) on the cells. The half maximal inhibitory concentration (IC50) value of 6G+ γ-T3 for HT-29 was 105 + 67 μg/mL and for SW837 it was 70 + 20 μg/mL. Apoptosis, active caspase-3 and annexin V FITC assays were performed after 24 h of treatment using flow cytometry. These bioactives in combination showed synergistic effect on HT-29 (CI: 0.89 ± 0.02,) and SW837 (CI: 0.79 ± 0.10) apoptosis was increased by 21.2% in HT-29 and 55.4% in SW837 (p

Original languageEnglish
Pages (from-to)10280-10297
Number of pages18
JournalMolecules
Volume20
Issue number6
DOIs
Publication statusPublished - 1 Jun 2015

Fingerprint

Tocotrienols
apoptosis
Cytotoxicity
Colorectal Neoplasms
activity (biology)
cancer
Cells
Apoptosis
Caspase 3
Assays
Flow cytometry
Fluorescein-5-isothiocyanate
Annexin A5
Inhibitory Concentration 50
cytometry
Flow Cytometry
gingerol
Neoplasms
cells

Keywords

  • 6-gingerol (6G)
  • HT-29
  • SW837 human colorectal cancer cells
  • Synergistic
  • γ-tocotrienol (γ-T3)

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

γ-Tocotrienol and 6-Gingerol in combination synergistically induce cytotoxicity and apoptosis in HT-29 and SW837 human colorectal cancer cells. / Yusof, Khairunnisa Md; Makpol, Suzana; A. Jamal, A. Rahman; Harun, Roslan; Mohd Mokhtar, Norfilza; Ngah, Wan Zurinah Wan.

In: Molecules, Vol. 20, No. 6, 01.06.2015, p. 10280-10297.

Research output: Contribution to journalArticle

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