γ-Glutamyl transpeptidase, glutathione S-transferase, alkaline phosphatase, and glutathione levels during different stages of chemically induced hepatocarcinogenesis in the rat

Suzana Makpol, Wan Zurinah Wan Ngah, Nor Aripin Shamaan, Zanariah Jarien, Alini Marzuki, Bin Abdul Kadir Khalid

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Abstract

Chemically induced hepatocarcinogenesis in rats with partial hepatectomy was followed morphologically and enzymatically at 4, 6, 8, 12, and 16 weeks after injection of the inducer, diethylnitrosamine. The enzymes determined were plasma and liver γ-glutamyltranspeptidase (GGT), alkaline phosphatase (ALP), and glutathione S-transferase (GST). The livers of the treated rats killed after 8 weeks appeared to be rough, pale, and larger compared with the control ones. After 6 weeks, large nodules were observed on the treated liver. Staining of the liver sections histochemically and immunohistochemically revealed that the enzyme-positive loci increased with time (r = 0.93, p < 0.05, for the placental form of GST (PGST); not significantly for GGT). The number of enzyme-positive foci per tissue surface area did not correlate with time. GGT, ALP, and GST activities in the plasma and liver of the treated rats were higher than those in the controls. Blood glutathione levels were not affected during chemically induced hepatocarcinogenesis in the rat.

Original languageEnglish
Pages (from-to)121-129
Number of pages9
JournalJournal of Clinical Biochemistry and Nutrition
Volume20
Issue number2
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

gamma-glutamyltransferase
gamma-Glutamyltransferase
Glutathione Transferase
glutathione transferase
Liver
Glutathione
Alkaline Phosphatase
alkaline phosphatase
Rats
glutathione
liver
rats
Enzymes
enzymes
Plasmas
Diethylnitrosamine
Hepatectomy
surface area
Blood
Tissue

Keywords

  • glutathione
  • hepatocarcinogenesis
  • histochemistry
  • marker enzymes
  • rat

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

γ-Glutamyl transpeptidase, glutathione S-transferase, alkaline phosphatase, and glutathione levels during different stages of chemically induced hepatocarcinogenesis in the rat. / Makpol, Suzana; Ngah, Wan Zurinah Wan; Shamaan, Nor Aripin; Jarien, Zanariah; Marzuki, Alini; Khalid, Bin Abdul Kadir.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 20, No. 2, 1996, p. 121-129.

Research output: Contribution to journalArticle

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